Single-Cell Survey of Human Lymphatics Unveils Marked Endothelial Cell Heterogeneity and Mechanisms of Homing for Neutrophils.

Autor:	Takeda A; MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland., Hollmén M; MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland., Dermadi D; Laboratory of Immunology and Vascular Biology, Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA., Pan J; Laboratory of Immunology and Vascular Biology, Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA; Veterans Affairs Palo Alto Health Care System and The Palo Alto Veterans Institute for Research, Palo Alto, CA, USA., Brulois KF; Laboratory of Immunology and Vascular Biology, Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA., Kaukonen R; Turku Centre for Biotechnology, University of Turku, Turku, Finland., Lönnberg T; Turku Centre for Biotechnology, University of Turku, Turku, Finland., Boström P; Department of Pathology, Turku University Hospital, Turku, Finland., Koskivuo I; Department of Plastic and General Surgery, Turku University Hospital, Turku, Finland., Irjala H; Department of Otorhinolaryngology, Turku University Hospital and University of Turku, Finland., Miyasaka M; MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland; Interdisciplinary Program for Biomedical Sciences, Institute for Academic Initiatives, Osaka University, Suita, Japan., Salmi M; MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland., Butcher EC; Laboratory of Immunology and Vascular Biology, Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA; Veterans Affairs Palo Alto Health Care System and The Palo Alto Veterans Institute for Research, Palo Alto, CA, USA., Jalkanen S; MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland. Electronic address: sirjal@utu.fi.
Jazyk:	angličtina
Zdroj:	Immunity [Immunity] 2019 Sep 17; Vol. 51 (3), pp. 561-572.e5. Date of Electronic Publication: 2019 Aug 08.
DOI:	10.1016/j.immuni.2019.06.027
Abstrakt:	Lymphatic vessels form a critical component in the regulation of human health and disease. While their functional significance is increasingly being recognized, the comprehensive heterogeneity of lymphatics remains uncharacterized. Here, we report the profiling of 33,000 lymphatic endothelial cells (LECs) in human lymph nodes (LNs) by single-cell RNA sequencing. Unbiased clustering revealed six major types of human LECs. LECs lining the subcapsular sinus (SCS) of LNs abundantly expressed neutrophil chemoattractants, whereas LECs lining the medullary sinus (MS) expressed a C-type lectin CD209. Binding of a carbohydrate Lewis X (CD15) to CD209 mediated neutrophil binding to the MS. The neutrophil-selective homing by MS LECs may retain neutrophils in the LN medulla and allow lymph-borne pathogens to clear, preventing their spread through LNs in humans. Our study provides a comprehensive characterization of LEC heterogeneity and unveils a previously undefined role for medullary LECs in human immunity. (Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu