Sublethal effects of microcystin-LR in the exposure and depuration time in a neotropical fish: Multibiomarker approach.

Autor: Calado SLM; Ecology and Conservation Program Post-Graduation, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: sabrinacalado@ufpr.br., Vicentini M; Ecology and Conservation Program Post-Graduation, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: maiaravicentini@ufpr.br., Santos GS; Department of Genetics, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: gustavosouza@ufpr.br., Pelanda A; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: anapelanda@ufpr.br., Santos H; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: hayanna.santos@ufpr.br., Coral LA; Department of Chemistry and Biology, Federal Technical University of Paraná, 81280-340, Curitiba-PR, Brazil. Electronic address: lucilacoral@utfpr.edu.br., Magalhães VF; Institute of Biophysics Carlos Chagas Filho, 21941-902, Ilha do Fundão, Rio de Janeiro, Brazil. Electronic address: valeria@biof.ufrj.br., Mela M; Department of Cell Biology, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: maritana.mela@ufpr.br., Cestari MM; Department of Genetics, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: margaces@ufpr.br., Silva de Assis HC; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-990, Curitiba-PR, Brazil. Electronic address: helassis@ufpr.br.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2019 Nov 15; Vol. 183, pp. 109527. Date of Electronic Publication: 2019 Aug 07.
DOI: 10.1016/j.ecoenv.2019.109527
Abstrakt: Eutrophication is an ecological process that results in cyanobacterial blooms. Microcystin-LR is the most toxic variant of microcystins and may cause toxic effects in the organisms, mainly in hepatic tissues. The aims of this study were to use multiple biomarkers in order to evaluate the sublethal effects of a low concentration of MC-LR (1 μg/L) in fish Geophagus brasiliensis by waterborne exposure; and evaluate the depuration of this toxin during 15 days. A group of 30 fish was exposed to 1 μg/L of MC-LR solution for 96 h in a static bioassay. After this time, blood, brain, muscle, liver, gonad and gills were collected from half of the exposed fish group in order to evaluate chemical, biochemical, histological and genotoxic biomarkers. The rest of the fish group was submitted to the depuration experiment with free MC-LR water for 15 days. After this time the same tissues were collected and evaluated using biomarkers analysis. Toxic effects were found mostly in the fish liver from depuration time as alterations on the antioxidant system and histopathologies. The results showed that even low concentrations can cause sublethal effects to aquatic organisms, and cyanotoxins monitoring and regulation tools are required.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: