Donor-specific B Cell Memory in Alloimmunized Kidney Transplant Recipients: First Clinical Application of a Novel Method.

Autor:	Wehmeier C; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Karahan GE; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Krop J; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., de Vaal Y; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Langerak-Langerak J; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Binet I; Department of Nephrology and Transplantation Medicine, Kantonsspital St. Gallen, St. Gallen, Switzerland., Schaub S; Clinic for Transplantation Immunology and Nephrology, University Hospital Basel, Basel, Switzerland., Roelen DL; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Claas FHJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands., Heidt S; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands.
Jazyk:	angličtina
Zdroj:	Transplantation [Transplantation] 2020 May; Vol. 104 (5), pp. 1026-1032.
DOI:	10.1097/TP.0000000000002909
Abstrakt:	Background: HLA-specific memory B cells may contribute to the serum HLA antibody pool upon antigen reexposure. The aim of this pilot study was to investigate the presence of concurrent donor-specific memory B cell-derived HLA antibodies (DSA-M) in renal allograft recipients with pretransplant donor-specific HLA antibodies (DSA) and its association with occurrence of antibody-mediated rejection (AMR) using a recently developed method. Methods: Twenty patients with Luminex single antigen bead (SAB) assay-defined DSA but negative complement-dependent cytotoxicity crossmatches were enrolled. Plasma samples and peripheral blood mononuclear cells were collected at 3 timepoints (pretransplant, mo 6, mo 12). We analyzed IgG-purified and concentrated culture supernatants from polyclonally activated peripheral blood mononuclear cells using SAB assays and compared HLA antibody profiles with same day plasma results. Results: Plasma SAB analysis revealed 35 DSA in 20 patients pretransplant. DSA-M were detected in 9 of 20 (45%) patients and for 10 of 35 specificities (29%). While median mean fluorescence intensity values of DSA with concurrent DSA-M (5877) were higher than those of DSA without DSA-M (1476), 3 of 6 patients with AMR and low mean fluorescence intensity DSA (<3000) had DSA-M. Overall, pretransplant DSA/DSA-Mpos allograft recipients showed a higher incidence of biopsy-proven (sub)clinical AMR (P = 0.032) and a higher extent (g≥1 + ptc≥1) of microvascular inflammation (67% vs 9%, P = 0.02). In 17 patients (28 DSA) with posttransplant analyses, persisting DSA posttransplant had more often DSA-M (6/12; 50%) than nonpersisting DSA (2/16; 13%). Conclusions: Assessment of DSA-M might be a novel tool to supplement serum HLA antibody analysis for pretransplant risk stratification in patients with DSA.
Databáze:	MEDLINE
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