Development of dairy herd of transgenic goats as biofactory for large-scale production of biologically active recombinant human lactoferrin.

Autor: Semak I; Belarusian State University, Nezavisimosti Avenue 4, 220030, Minsk, Belarus. semak@bsu.by., Budzevich A; Scientific and Practical Centre on Animal Husbandry of the National Academy of Sciences of Belarus, 11 Frunze Str., 222160, Zhodino, Belarus., Maliushkova E; Belarusian State University, Nezavisimosti Avenue 4, 220030, Minsk, Belarus., Kuzniatsova V; Scientific and Practical Centre on Animal Husbandry of the National Academy of Sciences of Belarus, 11 Frunze Str., 222160, Zhodino, Belarus., Popkov N; Scientific and Practical Centre on Animal Husbandry of the National Academy of Sciences of Belarus, 11 Frunze Str., 222160, Zhodino, Belarus., Zalutsky I; Institute of Physiology of the National Academy of Sciences of Belarus, 28 Academicheskaya Str., 220072, Minsk, Belarus., Ivashkevich O; Belarusian State University, Nezavisimosti Avenue 4, 220030, Minsk, Belarus.
Jazyk: angličtina
Zdroj: Transgenic research [Transgenic Res] 2019 Dec; Vol. 28 (5-6), pp. 465-478. Date of Electronic Publication: 2019 Aug 08.
DOI: 10.1007/s11248-019-00165-y
Abstrakt: The primary male-goats Lac-1 (human lactoferrin gene construct hLF5) and Lac-2 (human lactoferrin gene construct hLF3) with genome containing human lactoferrin gene were bred and the sperm bank of primary male-goats and their male descendents (F 1 -F 7 ) was created. The herd of goats (200 transgenic females) that produced recombinant human lactoferrin (rhLF) in their milk at levels up to 16 g/L was obtained. The rhLF from milk of transgenic goats, natural human lactoferrin (hLF) from woman milk and natural goat lactoferrin (gLF) from milk of non-transgenic goats were purified using cation-exchange chromatography. It has been shown that rhLF is a glycoprotein and its physicochemical characteristics of rhLF are similar to hLf as revealed by different analytical methods including electron paramagnetic resonance, spectrophotometry, differential scanning calorimetry, mass spectrometry and peptide mapping. The high expression level of rhLF achieved in milk of transgenic goats provides a solid basis for developing an efficient and cost-effective downstream processing. The rhLF exhibited a prominent biological activity suggesting it as a promising biopharmaceutical and food supplements.
Databáze: MEDLINE
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