Antioxidant Effects of Oral Ang-(1-7) Restore Insulin Pathway and RAS Components Ameliorating Cardiometabolic Disturbances in Rats.

Autor: Figueiredo VP; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., Barbosa MA; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., de Castro UGM; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., Zacarias AC; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., Bezerra FS; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.; Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Brazil., de Sá RG; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.; Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Brazil., de Lima WG; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.; Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Brazil., Dos Santos RAS; Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Alzamora AC; NUPEP, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.; Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Brazil.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2019 Jul 14; Vol. 2019, pp. 5868935. Date of Electronic Publication: 2019 Jul 14 (Print Publication: 2019).
DOI: 10.1155/2019/5868935
Abstrakt: In prevention studies of metabolic syndrome (MetS), Ang-(1-7) has shown to improve the insulin signaling. We evaluated the HP β CD/Ang-(1-7) treatment on lipid metabolism, renin-angiotensin system (RAS) components, oxidative stress, and insulin pathway in the liver and gastrocnemius muscle and hepatic steatosis in rats with established MetS. After 7 weeks of high-fat (FAT) or control (CT) diets, rats were treated with cyclodextrin (HP β CD) or HP β CD/Ang-(1-7) in the last 6 weeks. FAT-HP β CD/empty rats showed increased adiposity index and body mass, gene expression of ACE/ANG II/AT1R axis, and oxidative stress. These results were accompanied by imbalances in the insulin pathway, worsening of liver function, hyperglycemia, and dyslipidemia. Oral HP β CD/Ang-(1-7) treatment decreased ACE and AT1R , increased ACE2 gene expression in the liver, and restored thiobarbituric acid reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), insulin receptor substrate ( Irs-1 ), glucose transporter type 4 ( GLUT4 ), and serine/threonine kinase 2 ( AKT-2 ) gene expression in the liver and gastrocnemius muscle improving hepatic function, cholesterol levels, and hyperglycemia in MetS rats. Overall, HP β CD/Ang-(1-7) treatment restored the RAS components, oxidative stress, and insulin signaling in the liver and gastrocnemius muscle contributing to the establishment of blood glucose and lipid homeostasis in MetS rats.
Competing Interests: The authors declare no conflicting financial interests.
Databáze: MEDLINE
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