Metanephric organ culture.

Autor: Maser RL; Department of Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS, United States; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, United States; Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States. Electronic address: rmaser@kumc.edu., Magenheimer BS; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, United States; Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States., Calvet JP; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, United States; Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States.
Jazyk: angličtina
Zdroj: Methods in cell biology [Methods Cell Biol] 2019; Vol. 153, pp. 169-183. Date of Electronic Publication: 2019 Jun 13.
DOI: 10.1016/bs.mcb.2019.04.018
Abstrakt: Metanephric organ culture, or ex vivo embryonic kidney culture, was developed in the mid-twentieth century as a means to understand the development of the mammalian kidney and was used in early studies of polycystic kidney disease to explore mechanisms of renal cyst initiation by non-genetic factors. Following the identification of cystogenic genes, a resurgence of the use of metanephric organ culture occurred and has yielded insight into basic mechanisms of cystic dilation; facilitated identification of pathogenic pathways and potential therapeutic targets; and provided a system for evaluating therapeutic agents. This chapter provides detailed, step-by-step protocols with rationale and tips for the establishment, maintenance and treatment of metanephric organ cultures, and for performance of the most commonly employed secondary analyses of these cultures.
(© 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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