| Autor: |
Harorli OT; 1Department of Restorative Dentistry, Faculty of Dentistry, Akdeniz University, Antalya, Turkey., Hatipoglu M; 2Department of Periodontology, Faculty of Dentistry, Akdeniz University, Antalya, Turkey., Erin N; 3Department of Medical Pharmacology, School of Medicine, Akdeniz University, Antalya, Turkey. |
| Jazyk: |
angličtina |
| Zdroj: |
Photobiomodulation, photomedicine, and laser surgery [Photobiomodul Photomed Laser Surg] 2019 Aug; Vol. 37 (8), pp. 457-464. |
| DOI: |
10.1089/photob.2018.4589 |
| Abstrakt: |
Objective: The aim of this study was to evaluate the effects of 940-nm diode laser irradiation on proinflammatory cytokine secretions [interleukin (IL)-6 and IL-8] by human gingival fibroblasts in vitro . Background: Photobiomodulation has been routinely used in many dental procedures; however, the exact biological action mechanism of photobiomodulation and its therapeutic benefits have not been established. Methods: Cells derived from systemically healthy individuals were treated with three different laser parameters-6 J for 20 sec [0.84 J/cm 2 (0.04 W/cm 2 )], 10 J for 20 sec [1.4 J/cm 2 (0.07 W/cm 2 )], and 14 J for 20 sec [1.97 J/cm 2 (0.09 W/cm 2 )]-in the presence and absence of 1 μg/mL lipopolysaccharide (LPS) stimulation. Laser irradiations were carried out by a 940-nm diode laser device in continuous pain therapy mode with a deep tissue handpiece. Changes in cell viability, cytokine secretions, and mitogen-activated protein kinase pathway expressions were investigated, and results were compared with negative (medium) and positive control (1 μg/mL LPS) groups. The data obtained were statistically analyzed by the Mann-Whitney U test for pairwise comparisons among groups at the 0.05 level of significance. Results: Laser therapy with 0.84-1.4 J/cm 2 amplified IL-6 and IL-8 secretions, whereas 1.97 J/cm 2 suppressed IL-6 and IL-8 release in LPS-stimulated cells. Cell viability did not show a variation with photobiomodulation. Conclusions: These results demonstrate that photobiomodulation can alter IL-6 and IL-8 release, with cytokine suppression potency at a relatively high dose, as demonstrated previously. However, in contrast, we found that a low level of stimulation (6 J) in the presence of inflammation (LPS stimulation) may further enhance IL-6 and IL-8 release. We also found that p38 and ERK1/2 pathways are activated by LPS as well as by photobiomodulation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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