Endotracheal tube mucus as a source of airway mucus for rheological study.

Autor: Markovetz MR; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Subramani DB; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Kissner WJ; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Morrison CB; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Garbarine IC; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Ghio A; National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina., Ramsey KA; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Arora H; Department of Anesthesiology, University of North Carolina, Chapel Hill, North Carolina.; Outcomes Research Consortium, Cleveland, Ohio., Kumar P; Department of Anesthesiology, University of North Carolina, Chapel Hill, North Carolina.; Outcomes Research Consortium, Cleveland, Ohio., Nix DB; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia., Kumagai T; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia., Krunkosky TM; Department of Microbiology, University of Georgia, Athens, Georgia., Krause DC; Department of Microbiology, University of Georgia, Athens, Georgia., Radicioni G; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Alexis NE; Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, Chapel Hill, North Carolina., Kesimer M; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina.; Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina., Tiemeyer M; Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia., Boucher RC; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Ehre C; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina., Hill DB; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina.; Department of Physics and Astronomy, University of North Carolina, Chapel Hill, North Carolina.
Jazyk: angličtina
Zdroj: American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2019 Oct 01; Vol. 317 (4), pp. L498-L509. Date of Electronic Publication: 2019 Aug 07.
DOI: 10.1152/ajplung.00238.2019
Abstrakt: Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally. In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance. MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis. Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus. Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated. Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus. We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.
Databáze: MEDLINE