Grafting phenolics onto milk protein via conjugated polymerization for delivery of multiple functionalities: Synthesis and characterization.

Autor: El-Maksoud AAA; Department of Dairy Science, Faculty of Agriculture, Cairo University, Giza 12613, Egypt; Department of Engineering, Aarhus University, Gustav Wieds vej 10, Aarhus 8000, Denmark. Electronic address: ahmed_ali@cu.edu.eg., Anankanbil S; Department of Engineering, Aarhus University, Gustav Wieds vej 10, Aarhus 8000, Denmark. Electronic address: sampsonanankanbil@eng.au.dk., Zhou Y; Department of Engineering, Aarhus University, Gustav Wieds vej 10, Aarhus 8000, Denmark. Electronic address: yezhou@eng.au.dk., El-Ghany IHA; Department of Dairy Science, Faculty of Agriculture, Cairo University, Giza 12613, Egypt., El-Beltagi HS; Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Alhassa 31982, Saudi Arabia; Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. Electronic address: helbeltagi@kfu.edu.sa., Banerjee C; Department of Chemistry, Aarhus University, Gustav Wieds vej 14, Aarhus 8000, Denmark. Electronic address: banerjee@chem.au.dk., Petersen SV; Department of Biomedicine, Aarhus University, Wilhelm Meyers Allé 4, Aarhus 8000, Denmark. Electronic address: svp@biomed.au.dk., Guo Z; Department of Engineering, Aarhus University, Gustav Wieds vej 10, Aarhus 8000, Denmark. Electronic address: guo@eng.au.dk.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2019 Dec 15; Vol. 301, pp. 125298. Date of Electronic Publication: 2019 Jul 30.
DOI: 10.1016/j.foodchem.2019.125298
Abstrakt: A synthetic scenario for functionalization of β-lactoglobulin (βLg) with polymeric units containing caffeic acid (βLg-polyCA) was developed; and all intermediates and final products were structurally confirmed using nuclear magnetic resonance spectroscopy, matrix assisted laser desorption ionization time-of-flight mass spectrometry, and physico-chemically characterized using differential scanning calorimetry and circular dichroism. The antioxidant properties and emulsion stability of βLg, βLg-CA conjugate and βLg-polyCA based systems containing high percentage of fish oil (50%) were evaluated; and βLg-polyCA presented the highest antioxidant and free radical-scavenging activity based on DPPH, ABTS and HS scavenging assays (92.4, 87.92 and 67.35% respectively). Thiobarbituric acid (TBARS) test demonstrated that compared to native βLg, βLg-polyCA afford up 4-5 fold of inhibition of oxidative rancidity and displayed drastic secondary structure changes. Compared to native βLg based emulsions, βLg-polyCA had larger oil droplet sizes, stronger negative zeta potentials (-69.9 mv), narrower size distributions (PDI: 0.22) and less creaming index.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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