PLGA nanoparticles optimized by Box-Behnken for efficient encapsulation of therapeutic Cymbopogon citratus essential oil.

Autor: Almeida KB; Faculdade de Farmácia, Universidade Federal Fluminense, Brazil; Pós-graduação em Ciências Aplicadas a Produtos para Saúde, Universidade Federal Fluminense, Brazil., Ramos AS; Laboratório de Plantas Medicinais e Derivados, FarManguinhos, Fiocruz, Brazil., Nunes JBB; Faculdade de Farmácia, Universidade Federal Fluminense, Brazil., Silva BO; Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Brazil., Ferraz ERA; Faculdade de Farmácia, Universidade Federal Fluminense, Brazil., Fernandes AS; Departmento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Brazil., Felzenszwalb I; Departmento de Biofísica e Biometria, Universidade do Estado do Rio de Janeiro, Brazil., Amaral ACF; Laboratório de Plantas Medicinais e Derivados, FarManguinhos, Fiocruz, Brazil., Roullin VG; Laboratoire de Nanotechnologie Pharmaceutique, Faculté de Pharmacie, Université de Montréal, Canada., Falcão DQ; Faculdade de Farmácia, Universidade Federal Fluminense, Brazil; Pós-graduação em Ciências Aplicadas a Produtos para Saúde, Universidade Federal Fluminense, Brazil; Laboratoire de Nanotechnologie Pharmaceutique, Faculté de Pharmacie, Université de Montréal, Canada. Electronic address: deborah.quintanilha.falcao@umontreal.ca.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2019 Sep 01; Vol. 181, pp. 935-942. Date of Electronic Publication: 2019 Jun 05.
DOI: 10.1016/j.colsurfb.2019.06.010
Abstrakt: This study aimed to optimize Cymbopogon citratus essential oil loaded into PLGA-nanoparticles by investigating the effect of processing variables (sonication time, ultrasound power, and essential oil/polymer ratio) on encapsulation efficiency and particle mean hydrodynamic diameter using Box-Behnken design. Nanoparticles were prepared by an emulsification/solvent diffusion method and physicochemically characterized by FTIR, DSC and TGA/DTA. Cytotoxicity was evaluated in human HaCat keratinocytes by WST-1 and LDH assays. The optimized formulation had a hydrodynamic mean diameter of 277 nm, a polydispersity index of 0.18, a Zeta potential of -16 mV and an encapsulation efficiency of 73%. Nanoparticle characterization showed that only citral was incorporated in nanocarriers, with some amount adsorbed on their surface, and highlighted the potential in increasing the oil thermal stability. The drug release profile demonstrated a biphasic pattern with a substantial sustained release depending on diffusion from the polymeric matrix. Toxicity effects on cell viability of pure essential oil at low concentrations were significantly eliminated when encapsulated. Results revealed the ability of PLGA-nanoparticles to improve essential oil physicochemical characteristics, by controlling release and reducing toxicity, suggesting their potential use in pharmaceutical preparations.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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