The use of bacteriophage MS2 for the development and application of a virucide decontamination test method for porous and heavily soiled surfaces.

Autor: Wyrzykowska-Ceradini B; Jacobs Technology Inc., Tullahoma, TN, USA., Calfee MW; Decontamination and Consequence Management Division, National Homeland Security Research Center, Office of Research and Development, US Environmental Protection Agency (US EPA), Research Triangle Park, NC, USA., Touati A; Jacobs Technology Inc., Tullahoma, TN, USA., Wood J; Decontamination and Consequence Management Division, National Homeland Security Research Center, Office of Research and Development, US Environmental Protection Agency (US EPA), Research Triangle Park, NC, USA., Mickelsen RL; CBRN Consequence Management Advisory Division, Office of Land and Emergency Management, US EPA, Research Triangle Park, NC, USA., Miller L; Animal and Plant Health Inspection Service, US Department of Agriculture (USDA), Riverdale, MD, USA., Colby M; National Institute of Food and Agriculture, USDA, Washington, DC, USA., Slone C; Jacobs Technology Inc., Tullahoma, TN, USA., Gatchalian NG; Arcadis Inc., Research Triangle Park, NC, USA., Pongur SG; Jacobs Technology Inc., Tullahoma, TN, USA., Aslett D; Jacobs Technology Inc., Tullahoma, TN, USA.
Jazyk: angličtina
Zdroj: Journal of applied microbiology [J Appl Microbiol] 2019 Nov; Vol. 127 (5), pp. 1315-1326. Date of Electronic Publication: 2019 Aug 19.
DOI: 10.1111/jam.14406
Abstrakt: Aims: (i) To develop an analytical method for recovery and quantification of bacteriophage MS2-as a surrogate for foot-and-mouth disease virus-from complex porous surfaces, with and without the presence of laboratory-developed agricultural grime; (ii) to evaluate, with a 4-log dynamic range, the virucidal activity of common biocides for their ability to decontaminate surfaces and hence remediate facilities, following a foreign animal disease contamination incident.
Methods and Results: An analytical method was developed and optimized for MS2 recovery from simulated agricultural surfaces. The addition of Dey-Engley neutralizing broth to an extraction buffer improved MS2 viability in liquid extracts, with optimal analytical holding times determined as <8 to ≤24 h, depending on matrix. The recovery of MS2 from surface materials decreased in the order: nonporous reference material >grimed porous materials >nongrimed porous materials. In disinfectant testing, two spray applications of pAB were effective against MS2 (≥4-log reduction) on all operational-scale materials. Two per cent citric acid had limited effectiveness, with a ≥4-log reduction observed on a selected subset of grimed concrete samples.
Conclusions: Decontamination efficacy test results can be affected by surface characteristics, extraction buffer composition, analytical holding time and surface-specific organism survivability. Efficacy should be evaluated using a test method that reflects the environmental characteristics of the intended application.
Significance and Impact of the Study: The results of this study demonstrate the importance of analytical method verification tests for disinfectant testing prior to application in complex environments.
(© 2019 The Society for Applied Microbiology.)
Databáze: MEDLINE
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