| Autor: |
Nguyen HH; Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam., Nhu BLQ; Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam., Uyen NNP; Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam., Nguyen VT; Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam., Bui HT; Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam. |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of reproduction and development [J Reprod Dev] 2019 Oct 23; Vol. 65 (5), pp. 423-432. Date of Electronic Publication: 2019 Aug 04. |
| DOI: |
10.1262/jrd.2019-050 |
| Abstrakt: |
Historically, it had been widely accepted that the female mammalian ovary contained a limited number of oocytes that would reduce over time, without the possibility of replenishment. However, recent studies have suggested that female germline stem cells (FGSCs) could replenish the oocyte-pool in adults. The aim of this study was to isolate FGSCs from porcine ovaries and differentiate them into oocyte-like cells (OLCs). The FGSCs were successfully isolated from porcine ovarian tissue and cultured in vitro, in DMEM/F-12 medium supplemented with growth factors (EGF, FGF, GDNF, etc.) and a supplement (N21). These cells possessed spherical morphology and expressed specific germline characteristics (Vasa, Stella, Oct4, c-kit). By evaluating different conditions for in vitro differentiation of FGSCs, co-culturing the isolated FGSCs with MEF cells, under three-dimensional (3D) cell cultures, were shown to be optimal. FGSCs could successfully be differentiated into OLCs and reached about 70 µm in diameter, with a large number of surrounding somatic cells. Importantly, OLCs contained large nuclei, about 25-30 µm, with filamentous chromatin, similar to oocyte morphology, and expressed oocyte-specific markers (Gdf9, Zp2, SCP3, etc.) at the same level as oocytes. In conclusion, we successfully isolated FGSCs from porcine ovarian tissue and differentiated them into oocyte-like cells. This will provide a valuable model for studying a new, alternative source of oocytes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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