Transfection of microRNA-143 mimic could inhibit migration of HN-5 cells through down-regulating of metastatic genes.

Autor: Mesgarzadeh AH; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran; Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Aali M; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran; Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Farhadi F; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran; Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Noorolyai S; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Baghbani E; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Mohammadnejad F; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Baradaran B; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: behzad_im@yahoo.com.
Jazyk: angličtina
Zdroj: Gene [Gene] 2019 Oct 20; Vol. 716, pp. 144033. Date of Electronic Publication: 2019 Aug 01.
DOI: 10.1016/j.gene.2019.144033
Abstrakt: Oral squamous cell cancer (OSCC) is one of the causes of death worldwide. The purpose of this project was to define the restoring of microRNA-143 in HN-5 cells and discover molecular apparatuses responsible for the anticancer processes. Firstly, expression levels of miR-143, K-Ras, MMP9 and C-Myc were evaluated in OSCC tissues. Then, microRNA-143 was transfected into HN-5 cells. The cytotoxic effects of microRNA-143 on HN-5 cells were evaluated. To estimate the effects of microRNA-143 on cell migration, wound healing assay was done. The expression levels of microRNA-143, K-Ras, MMP9, C-Myc, ADAMTS and CXCR4 were evaluated via the qRT-PCR method. microRNA-143 mimic inhibited cell migration in HN-5 cell line. microRNA-143 mimic decreased K-Ras, MMP9, C-My, ADAMTS and CXCR4 gene expression. microRNA-143 can inhibit HN-5 cells migration in vitro by down-regulating the expression of invasion-linked genes. Hence, microRNA-143 can be a new diagnostic biomarker and new therapeutic target for OSCC.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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