Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats.

Autor: de Sousa LM; Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil., Dos Santos Alves JM; Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil., da Silva Martins C; Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil., Pereira KMA; Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil.; Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil., Goes P; Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil.; Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil., Gondim DV; Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil. delanegondim@yahoo.com.br.; Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil. delanegondim@yahoo.com.br.
Jazyk: angličtina
Zdroj: Inflammation research : official journal of the European Histamine Research Society ... [et al.] [Inflamm Res] 2019 Oct; Vol. 68 (10), pp. 889-900. Date of Electronic Publication: 2019 Aug 01.
DOI: 10.1007/s00011-019-01274-4
Abstrakt: Objective: To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ).
Materials and Methods: Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1.
Results: The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections.
Conclusion: Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.
Databáze: MEDLINE
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