The effect of neutral electrolyzed water as a disinfectant of eggshells artificially contaminated with Listeria monocytogenes .

Autor: Rivera-Garcia A; Facultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City Mexico., Santos-Ferro L; Facultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City Mexico., Ramirez-Orejel JC; Facultad de Medicina Veterinaria y Zootecnia, Department of Animal Nutrition and Biochemistry Universidad Nacional Autónoma de México (UNAM) Mexico City Mexico., Agredano-Moreno LT; Cell Nanobiology Laboratory, Department of Cell Biology. Faculty of Sciences Universidad Nacional Autónoma de México (UNAM) Mexico City Mexico., Jimenez-Garcia LF; Cell Nanobiology Laboratory, Department of Cell Biology. Faculty of Sciences Universidad Nacional Autónoma de México (UNAM) Mexico City Mexico., Paez-Esquiliano D; Facultad de Medicina Veterinaria y Zootecnia, Department of Physiology and Pharmacology Universidad Nacional Autónoma de México (UNAM) Mexico City Mexico., Andrade-Esquivel E; Departamento de Ingenieria Bioquímica Instituto Tecnologico de Celaya Guanajuato Mexico., Cano-Buendia JA; Facultad de Medicina Veterinaria y Zootecnia, Department of Microbiology and Immunology Universidad Nacional Autónoma de Mexico (UNAM) Mexico City Mexico.
Jazyk: angličtina
Zdroj: Food science & nutrition [Food Sci Nutr] 2019 Jun 14; Vol. 7 (7), pp. 2252-2260. Date of Electronic Publication: 2019 Jun 14 (Print Publication: 2019).
DOI: 10.1002/fsn3.1053
Abstrakt: Neutral electrolyzed water (NEW) was tested as a disinfectant against Listeria monocytogenes on the surface of table eggs. Eggs were collected from a single Bovans White flock and were exposed to L. monocytogenes . Artificially contaminated eggs were divided into three different treatment groups: NEW, 2% citric acid solution (CAS), and saline solution (SS). To evaluate the bactericidal effect, the Mexican norm for antimicrobial activity determination protocol was performed. The observed bactericidal effect was compared against those obtained from CAS and SS. Bacterial cells present on the eggshells were quantified. NEW exhibited a significantly higher bactericidal effect than CAS when evaluated on the surfaces of chicken eggshells (6.11 log 10 CFU/ml reduction in vitro and a 2.18 log 10 CFU/egg reduction on eggs vs. 1.06 log 10 CFU/ml in vitro reduction and 1.74 log 10 CFU/egg). Additionally, CAS was found to react with the carbonate egg shield, resulting in a loss of cuticle integrity. Mineral content of NEW-treated eggshells was similar to SS-treated eggshells; however, CAS-treated eggshells showed a significant decrease in phosphorous concentration compared to NEW treatment. In this study, we demonstrated the effect of NEW and CAS on the integrity of the L. monocytogenes wall using transmission electron microscopy. To the best of our knowledge, this is the first report of the effect of NEW against L. monocytogenes on eggshells. Our results show that NEW is a viable alternative solution for the disinfection of table eggs that does not affect the cuticle or shell.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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