| Autor: |
Voth R; I. Medizinische Klinik und Poliklinik, Universität, Mainz, West Germany., Rossol S, Hess G, Laubenstein HP, Meyer zum Büschenfelde KH, Schröder HC, Bachmann M, Reuter P, Müller WE |
| Jazyk: |
angličtina |
| Zdroj: |
Japanese journal of cancer research : Gann [Jpn J Cancer Res] 1988 May; Vol. 79 (5), pp. 647-55. |
| DOI: |
10.1111/j.1349-7006.1988.tb00035.x |
| Abstrakt: |
Avarol is a cytostatic and anti-human immunodeficiency virus (HIV) agent. In this study, the avarol caused induction of gamma-interferon (IFN-gamma) in buffy coat cells (human peripheral blood lymphocytes) is demonstrated by immunological and molecular biological techniques. IFN-gamma production was detected after a 24-hr incubation period with avarol; maximal production was obtained after 5 days in the presence of the optimal avarol concentration of 0.75 microgram/ml. Blotting experiments using human IFN-gamma cDNA and beta-actin cDNA containing plasmids showed that in the absence of avarol no IFN-gamma transcripts were present in lymphocytes. Already after a 24-hr incubation with avarol, IFN-gamma gene induction was detected, and maximal induction was found after a 5-day incubation period. The enhanced IFN-gamma production seems to be caused by a change at the transcriptional and/or post-transcriptional level, but not during subsequent nucleocytoplasmic transport of mRNA. This molecular event is specific, at least in relation to the expression of the beta-actin gene. Our studies demonstrate that avarol displays, besides its potential anti-tumor and anti-HIV activity, a potential immunomodulating effect. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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