| Autor: |
Gonçalves LM; Department of Dentistry, Post-Graduate Program in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., Madeira PLB; Department of Dentistry, Post-Graduate Program in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., Diniz RS; Department of Dentistry, Post-Graduate Program in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., Nonato RF; Department of Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., de Siqueira FSF; Department of Dentistry, Post-Graduate Program in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., de Sousa EM; Laboratory of Applied Microbiology, Post-Graduate Program in Microbial Biology, CEUMA University, Sao Luis, Maranhao, Brazil., Farias DCS; Department of Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil., Rocha FMG; Laboratory of Applied Microbiology, Post-Graduate Program in Microbial Biology, CEUMA University, Sao Luis, Maranhao, Brazil., Rocha CHL; Laboratory of Applied Microbiology, Post-Graduate Program in Microbial Biology, CEUMA University, Sao Luis, Maranhao, Brazil., Lago ADN; Graduate Program in Dentistry, Federal University of Maranhao. UFMA, Sao Luis, Maranhao, Brazil., Monteiro CA; Laboratory of Applied Microbiology, Post-Graduate Program in Microbial Biology, CEUMA University, Sao Luis, Maranhao, Brazil. |
| Abstrakt: |
This study aimed to investigate the effect of the n-butanol fraction of Terminalia catappa Linn., (FBuTC) on biofilm of Candida albicans and Candida glabrata , as well as changes in color and roughness of polymethyl methacrylate resin (PMMA). The susceptibility of C. albicans and C. glabrata to FBuTC was evaluated by means of the Minimum Inhibitory and Minimum Fungicidal Concentration (MIC and MFC). PMMA acrylic resin discs (N= 108) were fabricated. For the susceptibility tests, biofilms of C. albicans and C. glabrata were developed on discs for 48 h and immersed in phosphate-saline buffer solution (PBS), 1% sodium hypochlorite (SH 1%), or FBuTC at MIC, 5xMIC, or 10xMIC. For the color and roughness change tests, the discs were immersed in distilled water, SH 1%, or FBuTC in the concentrations of 0.25 mg/mL, 2.5 mg/mL, or 25.0 mg/mL. After 28 days of incubation, color change was evaluated by spectrophotometry and roughness, by using a profilometer. The biofilms were investigated by one-way ANOVA and, the color and roughness changes (two-way ANOVA and the Tukey test; α =0.05). For both MIC and MFC the value of 0.25 mg/mL of FBuTC was observed for the planktonic cells of C. albicans and C. glabrata . Exposure to FBuTC at 10xMIC had a significant effect on the biofilm of C. albicans , showing a reduction in cell counts when compared with PBS, ( p =0.001). For the biofilm of C. glabrata , the MIC was sufficient for significantly reducing the cell count ( p <0.001). No important changes in color and roughness of the acrylic resin were observed, even after 28 days, irrespective of the concentration of FBuTC used ( p >0.05). It could be concluded that the immersion of acrylic resin for dental prosthesis in FBuTC was effective in reducing the biofilms of C. albicans and C. glabrata without evidence of change in roughness and color of this substrate. |
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