A Composite Hydrogel Scaffold Permits Self-Organization and Matrix Deposition by Cocultured Human Glomerular Cells.

Autor: Tuffin J; Bristol Renal, Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS13 NY, UK., Burke M; Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, BS8 1FD, UK., Richardson T; Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, BS8 1FD, UK., Johnson T; UCB, Slough, SL1 3WE, UK., Saleem MA; Bristol Renal, Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS13 NY, UK., Satchell S; Bristol Renal, Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS13 NY, UK., Welsh GI; Bristol Renal, Translational Health Sciences, Bristol Medical School, University of Bristol, Bristol, BS13 NY, UK., Perriman A; School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2019 Sep; Vol. 8 (17), pp. e1900698. Date of Electronic Publication: 2019 Jul 30.
DOI: 10.1002/adhm.201900698
Abstrakt: 3D scaffolds provide cells with a spatial environment that more closely resembles that of in vivo tissue, when compared to 2D culture on a plastic substrate. However, many scaffolding materials commonly used in tissue engineering tend to exhibit anisotropic morphologies that exhibit a narrow range of fiber diameters and pore sizes, which do not recapitulate extracellular matrices. In this study, a fibrin hydrogel is formed within the interstitial spaces of an electrospun poly(glycolic) acid (PGA) monolith to generate a composite, bimodal scaffold for the coculture of kidney glomerular cell lines. This new scaffold exhibits multiple fiber morphologies, containing both PGA microfibers (14.5 ± 2 µm) and fibrin gel nanofibers (0.14 ± 0.09 µm), which increase the compressive Young's modulus beyond that of either of the constituents. The composite structure provides an enhanced 3D environment that increases proliferation and adhesion of immortalized human podocytes and glomerular endothelial cells. Moreover, the micro/nanoscale fibrous morphology promotes motility and reorganization of the glomerular cells into glomerulus-like structures, resulting in the deposition of organized collagen IV; the primary component of the glomerular basement membrane (GBM).
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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