Molecular Surveillance of Drug Resistance of Plasmodium falciparum Isolates Imported from Angola in Henan Province, China.

Autor: Zhou R; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Yang C; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Li S; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Zhao Y; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Liu Y; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Qian D; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Wang H; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Lu D; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China., Zhang H; Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, People's Republic of China zhwei69@163.com huangfang@nipd.chinacdc.com., Huang F; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, People's Republic of China zhwei69@163.com huangfang@nipd.chinacdc.com.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2019 Sep 23; Vol. 63 (10). Date of Electronic Publication: 2019 Sep 23 (Print Publication: 2019).
DOI: 10.1128/AAC.00552-19
Abstrakt: Angola was the main origin country for the imported malaria in Henan Province, China. Antimalarial drug resistance has posed a threat to the control and elimination of malaria. Several molecular markers were confirmed to be associated with the antimalarial drug resistance, such as pfcrt , pfmdr1 , pfdhfr , pfdhps , and K13. This study evaluated the drug resistance of the 180 imported Plasmodium falciparum isolates from Angola via nested PCR using Sanger sequencing. The prevalences of pfcrt C 72 V 73 M 74 N 75 K 76 , pfmdr1 N 86 Y 184 S 1034 N 1042 D 1246 , pfdhfr A 16 N 51 C 59 S 108 D 139 I 164 , and pfdhps S 436 A 437 A 476 K 540 A 581 were 69.4%, 59.9%, 1.3% and 6.3%, respectively. Three nonsynonymous (A578S, M579I, and Q613E) and one synonymous (R471R) mutation of K13 were found, the prevalences of which were 2.5% and 1.3%, respectively. The single nucleotide polymorphisms (SNPs) in pfcrt , pfmdr1 , pfdhfr , and pfdhps were generally shown as multiple mutations. The mutant prevalence of pfcrt reduced gradually, but pfdhfr and pfdhps still showed high mutant prevalence, while pfmdr1 was relatively low. The mutation of the K13 gene was rare. Molecular surveillance of artemisinin (ART) resistance will be used as a tool to evaluate the real-time efficacy of the artemisinin-based combination therapies (ACTs) and the ART resistance situation.
(Copyright © 2019 American Society for Microbiology.)
Databáze: MEDLINE
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