Developing Gut Microbiota Exerts Colonisation Resistance to Clostridium ( syn. Clostridioides) difficile in Piglets.

Autor: Grześkowiak Ł; Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise Strasse 49, 14195 Berlin, Germany. lukasz.grzeskowiak@fu-berlin.de., Dadi TH; Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise Strasse 49, 14195 Berlin, Germany., Zentek J; Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise Strasse 49, 14195 Berlin, Germany., Vahjen W; Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise Strasse 49, 14195 Berlin, Germany.
Jazyk: angličtina
Zdroj: Microorganisms [Microorganisms] 2019 Jul 26; Vol. 7 (8). Date of Electronic Publication: 2019 Jul 26.
DOI: 10.3390/microorganisms7080218
Abstrakt: Clostridium (syn. Clostridioides ) difficile is considered a pioneer colonizer and may cause gut infection in neonatal piglets. The aim of this study was to explore the microbiota- C. difficile associations in pigs. We used the DNA from the faeces of four sows collected during the periparturient period and from two to three of their piglets (collected weekly until nine weeks of age) for the determination of bacterial community composition (sequencing) and C. difficile concentration (qPCR). Furthermore, C. difficile -negative faeces were enriched in a growth medium, followed by qPCR to verify the presence of this bacterium. Clostridium -sensu-stricto-1 and Lactobacillus spp. predominated the gut microbiota of the sows and their offspring. C. difficile was detected at least once in the faeces of all sows during the entire sampling period, albeit at low concentrations. Suckling piglets harboured C. difficile in high concentrations (up to log 10 9.29 copy number/g faeces), which gradually decreased as the piglets aged. Enrichment revealed the presence of C. difficile in previously C. difficile -negative sow and offspring faeces. In suckling piglets, the C. difficile level was negatively correlated with carbohydrate-fermenting bacteria, and it was positively associated with potential pathogens. Shannon and richness diversity indices were negatively associated with the C. difficile counts in suckling piglets. This study showed that gut microbiota seems to set conditions for colonisation resistance against C. difficile in the offspring. However, this conclusion requires further research to include host-specific factors.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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