Negative interaction of Staphylococcus aureus on Fusarium falciforme growth ocular isolates in an in vitro mixed biofilm.

Autor: Bautista-Hernández LA; Microbiology and Ocular Proteomics Dept, Inst of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Mexico City, Mexico; Doctorate Biological Science and Health, Autonomus Metropolitan University, Mexico City, Mexico., Gómez-Olivares JL; Department of Health Sciences, Autonomous Metropolitan University, Mexico City, Mexico., Buentello-Volante B; Cellular and Tissue Biology, Research Unit, Institute of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Chimalpopoca, 14, CP 06800, Mexico City, Mexico., Dominguez-Lopez A; Cellular and Tissue Biology, Research Unit, Institute of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Chimalpopoca, 14, CP 06800, Mexico City, Mexico., Garfias Y; Cellular and Tissue Biology, Research Unit, Institute of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Chimalpopoca, 14, CP 06800, Mexico City, Mexico; Department of Biochemistry, Faculty of Medicine, Autonomus National University of Mexico, Av. Universidad 3000, CP 04510, Mexico City, Mexico., Acosta-García MC; Department of Reproduction of Biology, Autonomus Metropolitan University. México City, Mexico., Calvillo-Medina RP; Microbiology and Ocular Proteomics Dept, Inst of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Mexico City, Mexico., Bautista-de Lucio VM; Microbiology and Ocular Proteomics Dept, Inst of Ophthalmology 'Fundación de Asistencia Privada Conde de Valenciana', Mexico City, Mexico. Electronic address: vbautistal@institutodeoftalmologia.org.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2019 Oct; Vol. 135, pp. 103644. Date of Electronic Publication: 2019 Jul 24.
DOI: 10.1016/j.micpath.2019.103644
Abstrakt: The interactions between prokaryotes and eukaryotes are abundant in nature. These microorganisms also interact in the human body. Fungal-bacteria interactions are present in many diseases. In this study, we evaluated the microbial interaction of Fusarium falciforme and Staphylococcus aureus developing mixed biofilm in vitro. When both microorganisms grew up together the mixed biofilm biomass decreased than F. falciforme monobiofilm biomass. S. aureus was able to interact and form aggregates over the mycelium and conidia surface of F. falciforme. Our results suggest that S. aureus could bind to colloidal chitin. On another hand, the supernatants from S. aureus biofilm and S. aureus-F. falciforme presented an antifungal effect over F. falciforme biofilm formation. Finally we found that the pH had an inhibitory effect over fungal biofilm formation. We concluded that S. aureus can affect the F. falciforme growth negatively in mixed biofilm involving factors like pH, supernatants compounds, anchor to chitin, and bacterial viability.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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