Dodecamers derived from the crystal structure were found in the pre-crystallization solution of the transaminase from the thermophilic bacterium Thermobaculum terrenum by small-angle X-ray scattering.

Autor:	Marchenkova MA; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Konarev PV; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Rakitina TV; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation.; Shemyakin - Ovchinnikov Institute of Bioorganic Chemistry, Laboratory of Hormonal Regulation Proteins, Russian Academy of Sciences, Moscow, Russian Federation., Timofeev VI; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Boikova AS; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Dyakova YA; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Ilina KB; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Korzhenevskiy DA; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Yu Nikolaeva A; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Pisarevsky YV; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation., Kovalchuk MV; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russian Federation.; National Research Centre 'Kurchatov Institute', Moscow, Russian Federation.; St. Petersburg State University, St. Petersburg, Russian Federation.
Jazyk:	angličtina
Zdroj:	Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2020 Jul; Vol. 38 (10), pp. 2939-2944. Date of Electronic Publication: 2019 Aug 08.
DOI:	10.1080/07391102.2019.1649195
Abstrakt:	The pre-crystallization solution of the transaminase from Thermobaculum terrenum (TaTT) has been studied by small-angle X-ray scattering (SAXS). Regular changes in the oligomeric composition of the protein were observed after the addition of the precipitant. Comparison of the observed oligomers with the crystal structure of TaTT (PDB ID 6GKR) shows that dodecamers may act as building blocks in the growth of transaminase single crystals. Correlating of these results to the similar X-ray studies of other proteins suggests that SAXS may be a valuable tool for searching optimum crystallization conditions. AbbreviationSAXSsmall-angle X-ray scatteringTatransaminaseTaTTtransaminase from Thermobaculum terrenum PLPpyridoxal-5'-phosphateR-PEAR-(þ)-1-phenylethylamineBCATbranched-chain amino acid aminotransferaseDAATD-aminoacid aminotransferaseR-TAR-amine:pyruvate transaminaseCommunicated by Ramaswamy H. Sarma.
Databáze:	MEDLINE
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