Experimental Study of Genetic Constellation of Cold-Adapted Master Donor Viruses for Live Attenuated Influenza Vaccine Type B.

Autor: Krutikova EV; A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine, St. Petersburg, Russia. krutikova.iem@mail.ru., Stepanova EA; A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine, St. Petersburg, Russia., Kiseleva IV; A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine, St. Petersburg, Russia., Rudenko LG; A. A. Smorodintsev Department of Virology, Institute of Experimental Medicine, St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2019 Jul; Vol. 167 (3), pp. 384-387. Date of Electronic Publication: 2019 Jul 26.
DOI: 10.1007/s10517-019-04532-x
Abstrakt: We studied the constellation of genes encoding polymerase complex proteins of master donor viruses for Russian live attenuated influenza vaccine type B. Reassortants of the reserve attenuation donor B/Leningrad/14/17/55 with B/USSR/60/69 master donor virus currently used for manufacturing seasonal influenza vaccine were prepared and examined. Most reassortants obtained by the classical reassortment method inherited all genes from the B/Leningrad/14/17/55 virus except the gene encoding PB1 subunit of the polymerase complex. One reassortant was selected for further evaluation of the role of PB1 gene. Greater attenuation of the strain for experimental animals (mice) in comparison with the original strains was demonstrated. This indicates high degree of constellation of genes of cold-adapted master donor viruses and the important compensating role of amino acid substitutions in the PB1 protein of B/Leningrad/14/17/55 donor virus in preventing viral hyperattenuation.
Databáze: MEDLINE
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