| Autor: |
Gilliland WM Jr; Eshelman School of Pharmacy , University of North Carolina , Chapel Hill , North Carolina 27599 , United States., Prince HMA; Eshelman School of Pharmacy , University of North Carolina , Chapel Hill , North Carolina 27599 , United States.; School of Medicine , University of North Carolina , Chapel Hill , North Carolina 27599 , United States., Poliseno A; Eshelman School of Pharmacy , University of North Carolina , Chapel Hill , North Carolina 27599 , United States.; School of Medicine , University of North Carolina , Chapel Hill , North Carolina 27599 , United States., Kashuba ADM; Eshelman School of Pharmacy , University of North Carolina , Chapel Hill , North Carolina 27599 , United States.; School of Medicine , University of North Carolina , Chapel Hill , North Carolina 27599 , United States.; Center for AIDS Research , University of North Carolina , Chapel Hill , North Carolina 27599 , United States., Rosen EP; Eshelman School of Pharmacy , University of North Carolina , Chapel Hill , North Carolina 27599 , United States. |
| Abstrakt: |
Here, we assess infrared matrix assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) analysis of hair as a clinical tool for monitoring patient adherence to the antiretroviral maraviroc (MVC). A custom MATLAB-based algorithm has been developed to streamline data analysis and generate longitudinal profiles of drug incorporation along the length of hair strands. Hair strands from volunteers enrolled in a directly observed therapy study were analyzed by IR-MALDESI MSI and processed using this tool to characterize the profiles of single doses and a daily dose regimen of MVC. Single dose responses were 1.7 [1.1, 2.5] mm (median [range]) wide along the length of the hair and were detected in 8 out of 12 volunteers. Daily dose profiles capturing 28 days of continuous dosing were approximately 5 times the intensity of single dose profiles and 10.5 [7.0, 13] mm wide, corresponding to 1 month of hair growth. MVC ion abundance was observed in all 12 volunteers for the daily dosing period. Daily dosing profiles were consistent with a model of MVC accumulation in hair based on linear superposition of a single dose response, indicating the potential for prediction of daily drug-taking behavior based on deconvolution of a complex longitudinal profile in hair. |
