| Autor: |
Pei CD; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China., Zhang LL; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China., Fei PG; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.; Henan Key Laboratory of Biological Psychiatry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China., Gu SN; Department of General Medicine, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China., Li WQ; Henan Key Laboratory of Biological Psychiatry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.; Department of Psychosomatic Medicine, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453002, P.R. China., Mu JL; Henan Key Laboratory of Biological Psychiatry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.; Department of Psychosomatic Medicine, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453002, P.R. China., Wang XS; Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China., Wang WS; Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.; Tuberculosis Research Institute of Xinxiang Medical University, Weihui, Henan 453002, P.R. China., Zhang XY; Department of Psychiatry and Behavioral Sciences, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA., Zhang ZH; Department of Psychosomatic Medicine, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453002, P.R. China., Song JG; Department of Neurology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China. |
| Abstrakt: |
Aryl hydrocarbon receptor nuclear translocator protein 2 (ARNT2), a member of the basic helix-loop-helix superfamily of transcription factors, may serve a vital role in neuronal survival and cell proliferation via formation of heterodimers with hypoxia-inducible factor-1α. Previous studies indicated that ARNT2 levels were elevated in the brains of ischemic rats; however, the involvement of ARNT2 in post-stroke depression (PSD) rats is not well understood. Therefore, the present study aimed to investigate the levels of ARNT2 in the hippocampi of PSD rats, and to clarify the potential association between ARNT2 and behavioral performance. A PSD rat model was established by middle cerebral artery occlusion (MCAO) followed by a 4-week chronic unpredictable mild stress (CUMS) regimen. A sucrose preference test and open field test (OFT) were conducted, and body weight was measured. In addition, reverse transcription-polymerase chain reaction and immunohistochemistry were performed to measure ARNT expression. Results indicated that MCAO+CUMS rats had lower weight gain, consumed less sucrose and moved less compared with controls. Furthermore, the mRNA and protein levels of ARNT in MCAO+CUMS rats were increased compared with in controls. The sucrose preference index and horizontal movement distance in the OFT were positively correlated with ARNT mRNA level. Thus, from these findings it was suggested that ARNT2 may be positively associated with improvement of cognitive impairment, and therefore may be a potential target in PSD treatment. |
