| Autor: |
Tomilin NV, Barenfeld LS |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of radiation biology and related studies in physics, chemistry, and medicine [Int J Radiat Biol Relat Stud Phys Chem Med] 1979 Mar; Vol. 35 (3), pp. 253-63. |
| DOI: |
10.1080/09553007914550291 |
| Abstrakt: |
gamma-Endonuclease Y, an enzyme that hydrolyses phosphodiester bonds at alkalistable lesions in gamma-irradiated (N2, tris buffer) DNA, has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 19 000, induces single-strand breaks with 3'OH-5'PO4 termini and contains endonuclease activity towards DNA treated with 7-bromomethylbenz(a)anthracene. gamma-Endonuclease Y induces breaks in OsO4-treated poly(dA-dT) and apparently is specific towards gamma-ray-induced base lesions of the t' type. The complete excision repair of gamma-endonuclease Y substrate sites has been performed in vitro by gamma-endonuclease Y, DNA polymerase and ligase. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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