Genetic diversity and natural selection of transmission-blocking vaccine candidate antigens Pvs25 and Pvs28 in Plasmodium vivax Myanmar isolates.

Autor: Lê HG; Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea; BK21Plus Team for Anti-aging Biotechnology and Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju, 52727, Republic of Korea. Electronic address: gianglee291994@gmail.com., Kang JM; Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea; BK21Plus Team for Anti-aging Biotechnology and Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju, 52727, Republic of Korea. Electronic address: gjm9951001@hanmail.net., Jun H; Department of Tropical Medicine, Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, 22212, Republic of Korea. Electronic address: hojongi2@inha.ac.kr., Lee J; Department of Tropical Medicine, Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, 22212, Republic of Korea. Electronic address: jylee5492@gmail.com., Moe M; Department of Medical Research Pyin Oo Lwin Branch, Pyin Oo Lwin, Myanmar. Electronic address: myamoee.dmr@gmail.com., Thái TL; Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea; BK21Plus Team for Anti-aging Biotechnology and Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju, 52727, Republic of Korea. Electronic address: thailamcnshe.hua@gmail.com., Lin K; Department of Medical Research Pyin Oo Lwin Branch, Pyin Oo Lwin, Myanmar. Electronic address: dr.khinlin.dir@gmail.com., Myint MK; Department of Medical Research Pyin Oo Lwin Branch, Pyin Oo Lwin, Myanmar. Electronic address: dr.myintmoekyaw@gmail.com., Yoo WG; Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul, 06974, Republic of Korea. Electronic address: wonwill@cau.ac.kr., Sohn WM; Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea. Electronic address: wmsohn@gnu.ac.kr., Kim TS; Department of Tropical Medicine, Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, 22212, Republic of Korea. Electronic address: tongsookim@inha.ac.kr., Na BK; Department of Parasitology and Tropical Medicine, Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Republic of Korea; BK21Plus Team for Anti-aging Biotechnology and Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju, 52727, Republic of Korea. Electronic address: bkna@gnu.ac.kr.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2019 Oct; Vol. 198, pp. 105104. Date of Electronic Publication: 2019 Jul 20.
DOI: 10.1016/j.actatropica.2019.105104
Abstrakt: Transmission-blocking vaccines (TBVs) target the sexual stages of malarial parasites to interrupt or reduce the transmission cycle have been one of approaches to control malaria. Pvs25 and Pvs28 are the leading candidate antigens of TBVs against vivax malaria. In this study, genetic diversity and natural selection of the two TBV candidate genes in Plasmodium vivax Myanmar isolates were analyzed. The 62 Myanmar P. vivax isolates showed 9 and 19 different haplotypes for Pvs25 and Pvs28, respectively. The nucleotide diversity of Pvs28 was slightly higher than Pvs25, but not significant. Most amino acid substitutions observed in Myanmar Pvs25 and Pvs28 were concentrated at the EGF-2 and EGF-3 like domains. Major amino acid changes found in Myanmar Pvs25 and Pvs28 were similar to those reported in the global population, but novel amino acid substitutions were also identified. Negative selection was predicted in Myanmar Pvs25, whereas Pvs28 was under positive selection. Comparative analysis of global Pvs25 and Pvs28 suggests a substantial geographical difference between the Asian and American/African Pvs25 and Pvs28. The geographical genetic differentiation and the evidence for natural selection in global Pvs25 and Pvs28 suggest that the functional consequences of the observed polymorphism need to be considered for the development of effective TBVs based on the antigens.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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