Centromere Protein N Participates in Cellular Proliferation of Human Oral Cancer by Cell-Cycle Enhancement.

Autor:	Oka N; Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan., Kasamatsu A; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Endo-Sakamoto Y; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Eizuka K; Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan., Wagai S; Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan., Koide-Ishida N; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Miyamoto I; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Iyoda M; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Tanzawa H; Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan.; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan., Uzawa K; Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan.; Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan.
Jazyk:	angličtina
Zdroj:	Journal of Cancer [J Cancer] 2019 Jun 09; Vol. 10 (16), pp. 3728-3734. Date of Electronic Publication: 2019 Jun 09 (Print Publication: 2019).
DOI:	10.7150/jca.32281
Abstrakt:	Centromere protein N (CENP-N), an important member of the centromere protein family, is essential for kinetochore assembly and chromosome segregation; however, the relevance of CENP-N in cancers remains unknown. The aim of this study was to investigate CENP-N expression and its functional mechanisms in oral squamous cell carcinoma (OSCC). CENP-N expression was up-regulated significantly in vitro and in vivo in OSCCs. Overexpressed CENP-N was closely (p < 0.05) correlated with tumor growth using quantitative reverse transcriptase-polymerase chain reaction, immunoblot analysis, and immunohistochemistry. CENP-N knockdown (shCENP-N) cells showed depressed cellular proliferation by cell-cycle arrest at the G1 phase with up-regulation of p21 Cip1 and p27 Kip1 and down-regulation of cyclin D1, CDK2, and CDK4. Interestingly, we newly discovered that calcitriol (1, 25-dihydroxyvitamin D3) controlled the CENP-N expression level, leading to inhibition of tumor growth similar to shCENP-N cells. These results suggested that CENP-N plays a critical role in determining proliferation of OSCCs and that calcitriol might be a novel therapeutic drug for OSCCs by regulating CENP-N. Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
Databáze:	MEDLINE
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