Regulation of IL-24 in human oral keratinocytes stimulated with Tannerella forsythia.

Autor: Ko YK; Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea., An SJ; Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea., Han NY; Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon, Korea., Lee H; Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon, Korea., Choi BK; Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea.
Jazyk: angličtina
Zdroj: Molecular oral microbiology [Mol Oral Microbiol] 2019 Oct; Vol. 34 (5), pp. 209-218. Date of Electronic Publication: 2019 Aug 19.
DOI: 10.1111/omi.12265
Abstrakt: Interleukin-24 is a pleiotropic immunoregulatory cytokine and a member of the IL-20R subfamily of the IL-10 family. The aim of this study was to investigate the regulation of IL-24 in the human oral keratinocyte cell line HOK-16B following infection with Tannerella forsythia, a major periodontal pathogen. T. forsythia induced the expression of IL-24 mRNA and the secretion of glycosylated IL-24 in HOK-16B cells. Glycosylation of IL-24 is linked to its solubility and bioavailability. T. forsythia-stimulated reactive oxygen species (ROS) induced the expression of IL-24, which was regulated by IL-6. The ROS inhibitor N-acetylcysteine and MAPK inhibitors significantly reduced the expression of IL-6 and IL-24 induced by T. forsythia. Recombinant human IL-24 significantly enhanced the expression of IL-1α, IL-8, CXCL10, and MCP-1 in HOK-16B cells. Together, these results indicate that ROS, MAPKs, and IL-6 comprise the axis of IL-24 expression in HOK-16B cells stimulated with T. forsythia. Thus, IL-24 may be involved in inflammation in oral keratinocytes.
(© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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