Controlling magnesium corrosion and degradation-regulating mineralization using matrix GLA protein.

Autor: Hong D; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, PA, USA., Zaky SH; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, PA, USA., Chong R; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA., Lukashova L; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA., Beniash E; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, PA, USA., Verdelis K; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA., Witte F; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, PA, USA; Department of Prosthodontics, Geriatric Dentistry and Craniomandibular Disorders, Charité - Universitätsmedizin Berlin, Aßmannshauser Straße 4-6, 14197 Berlin, Germany., Sfeir C; The Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Department of Periodontics and Preventive Dentistry, Pittsburgh, PA, USA; Department of Oral Biology, University of Pittsburgh, Pittsburgh, PA, USA; The McGowan Institute for Regenerative Medicine, University of Pittsburgh, PA, USA. Electronic address: csfeir@pitt.edu.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2019 Oct 15; Vol. 98, pp. 142-151. Date of Electronic Publication: 2019 Jul 19.
DOI: 10.1016/j.actbio.2019.05.048
Abstrakt: Magnesium (Mg) alloys are embraced for their biodegradability and biocompatibility. However, Mg degrades spontaneously in the biological environment in vivo and in vitro, triggering deposition of calcium phosphate on the metal. Upon complete metal absorption, minerals remain in the tissue, which could lead to pathogenic calcification. Hence, our aims are to test the feasibility of matrix GLA protein (MGP) to locally inhibit Mg mineralization that is induced by metal alloy degradation. MGP is a small secretory protein that has been shown to inhibit soft tissue calcification. We exposed Mg to MGP, stably transfected into mammalian cells. Results showed that less calcium and phosphorous deposition on the Mg surface when MGP was present relative to when it was not. In the in vivo mouse intramuscular model conducted for 4 and 6 weeks, Mg rods were embedded in collagen scaffolds, seeded with cells overexpressing MGP. Microtomography, electron dispersive x-ray spectroscopy, and histology assessments revealed lower deposited mineral volume around Mg rods from the MGP group. Compared to other groups, higher volume loss after implantation was observed from the MGP group at both time points, indicating a higher corrosion rate without the protective mineral layer. This study is the first to our knowledge to demonstrate that local exposure to a biomolecule, such as MGP, can modulate the corrosion of Mg-based implants. These findings may have important implications for the future design of endovascular stents and orthopedic devices.
(Copyright © 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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