Stable contacts of naïve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo.
Autor: | Kozlovski S; a Department of Immunology, Weizmann Institute of Science , Rehovot , Israel., Atrakchi O; a Department of Immunology, Weizmann Institute of Science , Rehovot , Israel., Feigelson SW; a Department of Immunology, Weizmann Institute of Science , Rehovot , Israel., Shulman Z; a Department of Immunology, Weizmann Institute of Science , Rehovot , Israel., Alon R; a Department of Immunology, Weizmann Institute of Science , Rehovot , Israel. |
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Jazyk: | angličtina |
Zdroj: | Cell adhesion & migration [Cell Adh Migr] 2019 Dec; Vol. 13 (1), pp. 315-321. |
DOI: | 10.1080/19336918.2019.1644857 |
Abstrakt: | It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and -2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes. |
Databáze: | MEDLINE |
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