Potential benefits of polysaccharides derived from marine alga Ulva lactuca against hepatotoxicity and nephrotoxicity induced by thiacloprid, an insecticide pollutant.

Autor: Kammoun I; Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Sfax, Tunisia., Sellem I; Laboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Ben Saad H; Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Sfax, Tunisia., Boudawara T; Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, Sfax, Tunisia., Nasri M; Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Sfax, Tunisia., Gharsallah N; Laboratory of Biotechnology Applied on Culture Improvement of Plants, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia., Mallouli L; Laboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia., Amara IB; Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Sfax, Tunisia.
Jazyk: angličtina
Zdroj: Environmental toxicology [Environ Toxicol] 2019 Nov; Vol. 34 (11), pp. 1165-1176. Date of Electronic Publication: 2019 Jul 21.
DOI: 10.1002/tox.22818
Abstrakt: The present study aimed to evaluate the potential protective and antioxidant effect of polysaccharides (PS) extracted from Ulva lactuca against thiacloprid (THC) induced nephrotoxicity and hepatotoxicity. The antioxidant capacity of PS was tested in vitro using ABTS radical scavenging activity and plasmid DNA cleavage assays andin vivo on adult male rats treated for 30 days. Animals were allocated into four groups: control; THC (22.5 mg/kg); THC (22.5 mg/kg) + PS1 (100 mg/kg diet); and THC (22.5 mg/kg) + PS2 (200 mg/kg diet). The structural features of PS were determined by Fourier transformed infrared (FT-IR), UV absorption peak detection, high performance liquid chromatography (HPLC) and gel permeation chromatography, and also functional properties were investigated. Overall, results indicated that THC increased significantly malondialdehyde, advanced oxidation protein products, glutathione levels, which is correlated with severe histological and plasmatic biochemical injuries in both liver and kidney tissues. However, cotreatment PS induced a significant protective and healing affects against the nephrotoxicity and hepatotoxcity induced by THC.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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