Separation detection of hemoglobin and glycated hemoglobin fractions in blood using the electrochemical microfluidic channel with a conductive polymer composite sensor.

Autor: Mozammal Hossain MD; Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea., Moon JM; Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea., Gurudatt NG; Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea; Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, and Internal Medicine, Gil Medical Center, Gachon University, Incheon, 21565, Republic of Korea., Park DS; Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea., Choi CS; Korea Mouse Metabolic Phenotyping Center, Lee Gil Ya Cancer and Diabetes Institute, and Internal Medicine, Gil Medical Center, Gachon University, Incheon, 21565, Republic of Korea. Electronic address: cschoi@gachon.ac.kr., Shim YB; Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea; Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan, 46241, Republic of Korea. Electronic address: ybshim@pusan.ac.kr.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2019 Oct 01; Vol. 142, pp. 111515. Date of Electronic Publication: 2019 Jul 15.
DOI: 10.1016/j.bios.2019.111515
Abstrakt: Separation and detection of hemoglobin (Hb) and glycated hemoglobin fractions (HbA1c, HbAld 1+2 , HbAle, HbAld3a, HbAl a+b , HbA2, and HbAld3b) was performed using an electrochemical AC field modulated separation channel (EMSC) coupled with a sensor probe. The sensor was fabricated based on immobilization of a redox mediator on the poly(2,2':5',5″-terthiophene-3'-p-benzoic acid, pTTBA) and N,S-doped porous carbon (NSPC) nanocomposite. The different types of catalytic redox mediators such as Nile Blue (NB), toluidine blue O (TBO), and Neutral Red (NR) were evaluated to achieve the efficient detection. Of these, the NB-based sensor showed the best analytical signal for Hb and HbA1c, thus it was characterized using various electrochemical and surface analysis methods. After that, the sensor was coupled with the EMSC to achieve the separation detection of the Hb family. The frequency and amplitude of the AC electrical field applied onto the EMSC walls were the main driving forces for the separation and sensitive detection of the analytes. Under optimized conditions, linear dynamic ranges for Hb and HbA1c among their fractions were obtained between 1.0 × 10 -6 to 3.5 mM and 3.0 × 10 -6 to 0.6 mM with the detection limit of 8.1 × 10 -7 ± 3.0 × 10 -8 and 9.2 × 10 -7 ± 5 × 10 -8  mM, respectively. Interference effects of other biomolecules were also investigated and the clinical applicability of the device was evaluated by the determination of total Hb and % HbA1c in real human blood samples.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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