Autor: |
Slim A; National Gene Bank of Tunisia, Boulevard du Leader Yasser Arafat Z. I Charguia 1, Tunis 1080, Tunisia. amine_slim_inat@yahoo.fr., Piarulli L; SINAGRI S.r.l., Spin Off of the University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy., Chennaoui Kourda H; National Gene Bank of Tunisia, Boulevard du Leader Yasser Arafat Z. I Charguia 1, Tunis 1080, Tunisia., Rouaissi M; Biotechnology and Physiology Laboratory, National Agronomic Research Institute of Tunisia (INRAT), Hedi Karray Street, Ariana 2049, Tunisia., Robbana C; National Gene Bank of Tunisia, Boulevard du Leader Yasser Arafat Z. I Charguia 1, Tunis 1080, Tunisia., Chaabane R; Biotechnology and Physiology Laboratory, National Agronomic Research Institute of Tunisia (INRAT), Hedi Karray Street, Ariana 2049, Tunisia., Pignone D; Institute of Biosciences and Bioresources of the National Research Council (IBBR-CNR), Via Amendola 165/A, 70126 Bari, Italy., Montemurro C; Department of Soil, Plant and Food Sciences (DiSSPA), Sect. Genetics and Plant Breeding, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy., Mangini G; Department of Soil, Plant and Food Sciences (DiSSPA), Sect. Genetics and Plant Breeding, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy. giacomo.mangini@uniba.it. |
Abstrakt: |
The Tunisian durum wheat germplasm includes modern cultivars and traditional varieties that are still cultivated in areas where elite cultivars or intensive cultivation systems are not suitable. Within the frame of a collection program of the National Gene Bank of Tunisia (NGBT), durum wheat germplasm was collected from different Tunisian agro-ecological zones. The collected samples were studied using simple sequence repeats (SSRs) markers to explore the genetic diversity and evaluate the genetic structure in Tunisian germplasm. The results demonstrated significant diversity in the Tunisian durum wheat germplasm, with clear differentiation between traditional varieties and modern cultivars. The population structure analysis allowed the identification of five subpopulations, two of which appear to be more strongly represented in germplasm collected in central and southern Tunisia, where environmental conditions at critical development phases of the plant are harsher. Moreover these subpopulations are underrepresented in modern varieties, suggesting that traits of adaptation useful for breeding more resilient varieties might be present in central and southern germplasm. Moreover, our results will support, the activity of in situ on farm conservation of Tunisian durum wheat germplasm started by the National Gene Bank of Tunisia along with the ex situ approach. |