| Autor: |
Valencia Ayala E; Universidad de San Martin de Porres, Facultad de Medicina Humana, Instituto de Investigación, Centro de Investigación en Infectología e Inmunología, Centro de Investigación de Medicina Tradicional y Farmacología, Avenida El Corregidor N°1531, Las Viñas, La Molina,Lima, Peru., Marcos Carbajal P; Universidad Peruana Unión, Universidad de San Martin de Porres, Escuela Profesional de Medicina Humana, Laboratorio de Investigación en Biología Molecular, Facultad de Medicina Humana, Instituto de Investigación, Centro de Investigación de Medicina de Altura, Centro de Investigación de Medicina Tradicional y Farmacología, Km 19 Carretera Central, Ñaña, Lurigancho, Avenida El Corregidor N°1531, Las Viñas, La MolinaLima, Peru., Coelho EB; Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Clinica Médica, Laboratório de Hipertensao Expermental e Farmacogenetica, Av. Bandeirantes, 3900 - Vila Monte Alegre, Ribeirão Preto - SP, 14049-900São Paulo, Brazil., Sandoval JS; Universidad de San Martin de Porres, Facultad de Medicina Humana, Instituto de Investigación, Centro de Investigación de Genética y Biología Molecular, Avenida El Corregidor N°1531, Las Viñas, La MolinaLima, Peru., Salazar Granara A; Universidad de San Martin de Porres, Facultad de Medicina Humana, Instituto de Investigación, Centro de Investigación de Medicina de Altura, Centro de Investigación de Medicina Tradicional y Farmacología, Av. Alameda del Corregidor 1531, La Molina, Lima CP 15026, Peru, Phone: (051) (01) 3652300 extension 151. |
| Abstrakt: |
Background The MDR1 gene presents several genetic polymorphisms with pharmacological implications. Therefore, the aim of the present study is to establish the genotype and allele frequencies of 3435C>T polymorphism of MDR1 gene into Peruvian populations (Coastal, Andean and Amazonian ecoregions), even considering the altitude (lowland <2500 m and highland >2500 m). Methods The polymorphism was analyzed by TaqMan genotyping assays in a group of 181 healthy unrelated Peruvian individuals. The comparison of genotype and allele frequencies of 3435C>T polymorphism was made with the Pearson test (X2), and, to calculate the genotype distributions, the Hardy-Weinberg equilibrium (HWE) was used. Results In all populations evaluated in this study, the genotype frequency distributions met HWE assumptions. The comparison between genotype and allele frequencies showed significant differences (p < 0.05), when the Andean, Coastal and Amazonian populations were compared. Also, significant differences (p < 0.05) were obtained when these populations were compared considering their altitudes. Likewise, in comparison with countries like USA, Finland, Nigeria and Kenya, the results showed significant differences (p < 0.05). Conclusions This investigation allowed us to establish the genotype and allele frequencies of 3435C>T polymorphism in different Peruvian populations, considering the geographic localization and even the altitude. |
