Soluble HLA-G levels in seminal plasma are associated with HLA-G 3'UTR genotypes and haplotypes.

Autor: Craenmehr MHC; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Haasnoot GW; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Drabbels JJM; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Spruyt-Gerritse MJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Cao M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., van der Keur C; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Kapsenberg JM; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Uyar-Mercankaya M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., van Beelen E; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Meuleman T; Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, The Netherlands., van der Hoorn MP; Department of Obstetrics and Gynaecology, Leiden University Medical Center, Leiden, The Netherlands., Heidt S; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Claas FHJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Eikmans M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: HLA [HLA] 2019 Oct; Vol. 94 (4), pp. 339-346. Date of Electronic Publication: 2019 Aug 08.
DOI: 10.1111/tan.13628
Abstrakt: Soluble HLA-G (sHLA-G) levels in human seminal plasma (SP) can be diverse and may affect the establishment of maternal-fetal tolerance and thereby the outcome of pregnancy. We investigated whether sHLA-G levels in SP are associated with polymorphisms in the 3'-untranslated region (UTR) and UTR haplotypes of the HLA-G gene. Furthermore, we compared the HLA-G genotype distribution and sHLA-G levels between men, whose partner experienced unexplained recurrent miscarriage (RM), and controls. Soluble HLA-G levels (n = 156) and HLA-G genotyping (n = 176) were determined in SP samples. The concentration of sHLA-G was significantly associated with several single-nucleotide polymorphisms (SNPs): the 14 base pair (bp) insertion/deletion (indel), +3010, +3142, +3187, +3196, and + 3509. High levels of sHLA-G were associated with UTR-1 and low levels with UTR-2, UTR-4, and UTR-7 (P < .0001). HLA-G genotype distribution and sHLA-G levels in SP were not significantly different between the RM group (n = 44) and controls (n = 31). In conclusion, seminal sHLA-G levels are associated with both singular SNPs and 3UTR haplotypes. HLA-G genotype and sHLA-G levels in SP are not different between men whose partner experienced RM and controls, indicating that miscarriages are not solely the result of low sHLA-G levels in SP. Instead, it is more likely that these miscarriages are the result of a multifactorial immunologic mechanism, whereby the HLA-G 3'UTR 14 bp ins/ins genotype plays a role in a proportion of the cases. Future studies should look into the functions of sHLA-G in SP and the consequences of low or high levels on the chance to conceive.
(© 2019 The Authors. HLA: Immune Response Genetics published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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