A single-step isolation by centrifugal partition chromatography of the potential anti-inflammatory glaucolide B from Lepidaploa chamissonis.

Autor: Da Silva LAL; Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil., Sandjo LP; Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil., Fratoni E; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil., Kinoshita Moon YJ; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil., Dalmarco EM; Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil., Biavatti MW; Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-900, Brazil. Electronic address: maique.biavatti@ufsc.br.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2019 Nov 08; Vol. 1605, pp. 460362. Date of Electronic Publication: 2019 Jul 12.
DOI: 10.1016/j.chroma.2019.460362
Abstrakt: Sesquiterpene lactones (SL) are commonly found in Asteraceae and present a promising anti-inflammatory activity. Previously described in Lepidaploa genus, glaucolide B has never been investigated for its anti-inflammatory potential. This study aimed to establish an efficient process for the extraction of glaucolide B (1) from Lepidaploa chamissonis leaves and to develop a simple and fast method for its purification by using centrifugal partition chromatography (CPC), as well as to investigate in vitro the anti-inflammatory effects of glaucolide B. Thus, an optimized washing extractive process performed on L. chamissonis leaves allowed to obtain a SL enriched extract (4.11 g). After a successful defatting pretreatment of the crude extract, the glaucolide B enriched ethyl acetate portion (2.00 g) was fractionated by CPC affording, in a single-step isolation, compound 1 (1.04 g) in great yield (25%) and purity (97%). Cytotoxicity effect of 1 on RAW 264.7 macrophages was determined by using MTT assay, revealing a CC 10 of 14.11 μM. Compound 1 at 1, 3 and 10 μM inhibited the nitrite/nitrate (NO x ) metabolites production and the pro-inflammatory interleukin 6 (IL-6) secretion on lipopolysaccharide-stimulated RAW 264.7 cells. The extractive process used turned to be selective for SL and CPC technique proved a simple and effective tool for the isolation of 1 within few hours. Isolated for the first time from L. chamissonis leaves, glaucolide B presented a significant inhibitory effect on both NO and IL-6 secretion under non-toxic concentrations.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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