Caveolin-1 Endows Order in Cholesterol-Rich Detergent Resistant Membranes.

Autor: Raggi C; National Center for Control and Evaluation of Medicines, Istituto Superiore di Sanità, 00161 Rome, Italy., Diociaiuti M; National Center for Rare Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy., Caracciolo G; Department of Molecular Medicine, 'La Sapienza' University, 00161 Rome, Italy., Fratini F; Scientific Service for Core Facilities, Istituto Superiore di Sanità, 00161 Rome, Italy., Fantozzi L; Present address : ARPALAZIO, Via Salaria per L'Aquila 6/8, 02100 Rieti, Italy., Piccaro G; Notified Body, Istituto Superiore di Sanità, 00161 Rome, Italy., Fecchi K; Reference Centre for Gender Medicine, Istituto Superiore di Sanità, 00161 Rome, Italy., Pizzi E; Scientific Service for Core Facilities, Istituto Superiore di Sanità, 00161 Rome, Italy., Marano G; Reference Centre for Gender Medicine, Istituto Superiore di Sanità, 00161 Rome, Italy., Ciaffoni F; Scientific Service for Core Facilities, Istituto Superiore di Sanità, 00161 Rome, Italy., Bravo E; Scientific Service for Research Coordination and Support, Istituto Superiore di Sanità, 00161 Rome, Italy., Fiani ML; National Center for Global Health, Istituto Superiore di Sanità, 00161 Rome, Italy. maria.fiani@iss.it., Sargiacomo M; National Center for Global Health, Istituto Superiore di Sanità, 00161 Rome, Italy. massimo.sargiacomo@iss.it.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2019 Jul 17; Vol. 9 (7). Date of Electronic Publication: 2019 Jul 17.
DOI: 10.3390/biom9070287
Abstrakt: Cholesterol-enriched functional portions of plasma membranes, such as caveolae and rafts, were isolated from lungs of wild-type (WT) and caveolin-1 knockout (Cav-1 KO) mice within detergent resistant membranes (DRMs). To gain insight into their molecular composition we performed proteomic and lipid analysis on WT and Cav-1 KO-DRMs that showed predicted variations of proteomic profiles and negligible differences in lipid composition, while Langmuir monolayer technique and small and wide-angle X-ray scattering (SAXS-WAXS) were here originally introduced to study DRMs biophysical association state. Langmuir analysis of Cav-1 containing DRMs displayed an isotherm with a clear-cut feature, suggesting the coexistence of the liquid-ordered ( L o ) phase typical of the raft structure, namely "cholesterol-rich L o phase," with a phase fully missing in Cav-1 KO that we named "caveolin-induced L o phase." Furthermore, while the sole lipid component of both WT and KO-DRMs showed qualitatively similar isotherm configuration, the reinsertion of recombinant Cav-1 into WT-DRMs lipids restored the WT-DRM pattern. X-ray diffraction results confirmed that Cav-1 causes the formation of a "caveolin-induced L o phase," as suggested by Langmuir experiments, allowing us to speculate about a possible structural model. These results show that the unique molecular link between Cav-1 and cholesterol can spur functional order in a lipid bilayer strictly derived from biological sources.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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