| Autor: |
Li Y; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Zhou XH; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Chen ZH; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Dai LL; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Cui CX; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Wu HL; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Wei QY; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Fan KM; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China., Xu YL; Department of Otorhinolaryngology, the Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China. |
| Abstrakt: |
Objective: To compare the carcinogenic abilities of CD133(+)CD44(+) laryngeal cancer stem cells and general laryngeal cancer stem cells and to identify the mechanism underlying the action of miRNAs. Methods: Solid tumor-derived laryngeal carcinoma stem cells and Hep-2-derived laryngeal carcinoma stem cells were cultured, and CD133(+)CD44(+) laryngeal cancer stem cells were sorted by flow cytometry. Boden chamber invasion assay, cell migration assay and tumor formation assay were then performed to compare the invasion, migration and tumorigenic abilities of CD133(+)CD44(+) laryngeal cancer stem cells and general laryngeal cancer stem cells. And then, miRNAs isolated from two laryngeal cancer stem cells were detected and analysed with miRNA chip. Results: (1)In Boyden chamber invasion assay, the cell invasion rate of CD133(+)CD44(+) laryngeal cancer stem cells was obviously higher (80.2%±2.3% vs. 63.9%±3.2%, t =5.011, P= 0.027); (2)CD133(+)CD44(+) laryngeal cancer stem cells also had higher mobility in cell migration assay (82.9%±1.1% vs. 70.9%±0.6%, t =4.514, P= 0.031); (3)In tumor formation assay, the tumor formation rate of CD133(+)CD44(+) laryngeal cancer stem cells was also higher (80% vs. 50%). What's more, we identified 15 miRNAs that were significantly upregulated in CD133(+)CD44(+) laryngeal cancer stem cells and 3 miRNAs that were significantly downregulated in CD133(+)CD44(+) laryngeal cancer stem cells, compared with normal laryngeal cancer stem cells. Conclusions: CD133(+)CD44(+) laryngeal cancer stem cells have stronger invasion, migration and tumorigenic abilities compared with normal laryngeal cancer stem cells, and the difference of miRNAs' expression is one of the possible causes. |
