Label-free time- and space-resolved exometabolite sampling of growing plant roots through nanoporous interfaces.

Autor: Patabadige DEW; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA., Millet LJ; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA., Aufrecht JA; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA.; The Bredesen Center, University of Tennessee-Knoxville, Knoxville, TN, USA., Shankles PG; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA.; The Bredesen Center, University of Tennessee-Knoxville, Knoxville, TN, USA., Standaert RF; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA.; Shull Wollan Center, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA., Retterer ST; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA. rettererst@ornl.gov.; The Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA. rettererst@ornl.gov.; The Bredesen Center, University of Tennessee-Knoxville, Knoxville, TN, USA. rettererst@ornl.gov., Doktycz MJ; Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA. doktyczmj@ornl.gov.; The Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN, 37831-6445, USA. doktyczmj@ornl.gov.; The Bredesen Center, University of Tennessee-Knoxville, Knoxville, TN, USA. doktyczmj@ornl.gov.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Jul 16; Vol. 9 (1), pp. 10272. Date of Electronic Publication: 2019 Jul 16.
DOI: 10.1038/s41598-019-46538-5
Abstrakt: Spatial and temporal profiling of metabolites within and between living systems is vital to understanding how chemical signaling shapes the composition and function of these complex systems. Measurement of metabolites is challenging because they are often not amenable to extrinsic tags, are diverse in nature, and are present with a broad range of concentrations. Moreover, direct imaging by chemically informative tools can significantly compromise viability of the system of interest or lack adequate resolution. Here, we present a nano-enabled and label-free imaging technology using a microfluidic sampling network to track production and distribution of chemical information in the microenvironment of a living organism. We describe the integration of a polyester track-etched (PETE) nanofluidic interface to physically confine the biological sample within the model environment, while allowing fluidic access via an underlying microfluidic network. The nanoporous interface enables sampling of the microenvironment above in a time-dependent and spatially-resolved manner. For demonstration, the diffusional flux through the PETE membrane was characterized to understand membrane performance, and exometabolites from a growing plant root were successfully profiled in a space- and time-resolved manner. This method and device provide a frame-by-frame description of the chemical environment that maps to the physical and biological characteristics of the sample.
Databáze: MEDLINE
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