A Coevolved EDS1-SAG101-NRG1 Module Mediates Cell Death Signaling by TIR-Domain Immune Receptors.

Autor:	Lapin D; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Kovacova V; Cellular Networks and Systems Biology, CECAD, University of Cologne, Cologne 50931, Germany.; Faculty of Statistical Physics of Biological Systems, Predictive Models of Evolution, Institute for Biological Physics, University of Cologne, Cologne 50937, Germany., Sun X; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Dongus JA; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Bhandari D; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., von Born P; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Bautor J; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Guarneri N; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Rzemieniewski J; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany., Stuttmann J; Department of Genetics, Institute for Biology, Martin Luther University Halle-Wittenberg, Halle 06120, Germany., Beyer A; Cellular Networks and Systems Biology, CECAD, University of Cologne, Cologne 50931, Germany., Parker JE; Department of Plant-Microbe Interactions, Max-Planck Institute for Plant Breeding Research, 50829 Cologne, Germany parker@mpipz.mpg.de.; Cologne-Düsseldorf Cluster of Excellence in Plant Sciences (CEPLAS) D-40225 Düsseldorf, Germany.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2019 Oct; Vol. 31 (10), pp. 2430-2455. Date of Electronic Publication: 2019 Jul 16.
DOI:	10.1105/tpc.19.00118
Abstrakt:	Plant nucleotide binding/leucine-rich repeat (NLR) immune receptors are activated by pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain NLRs (TNLs) converge on the ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) family of lipase-like proteins for all resistance outputs. In Arabidopsis ( Arabidopsis thaliana ) TNL-mediated immunity, At EDS1 heterodimers with PHYTOALEXIN DEFICIENT4 ( At PAD4) transcriptionally induced basal defenses. At EDS1 uses the same surface to interact with PAD4-related SENESCENCE-ASSOCIATED GENE101 ( At SAG101), but the role of At EDS1- At SAG101 heterodimers remains unclear. We show that At EDS1- At SAG101 functions together with N REQUIRED GENE1 ( At NRG1) coiled-coil domain helper NLRs as a coevolved TNL cell death-signaling module. At EDS1- At SAG101- At NRG1 cell death activity is transferable to the Solanaceous species Nicotiana benthamiana and cannot be substituted by At EDS1- At PAD4 with At NRG1 or At EDS1- At SAG101 with endogenous Nb NRG1. Analysis of EDS1-family evolutionary rate variation and heterodimer structure-guided phenotyping of At EDS1 variants and At PAD4- At SAG101 chimeras identify closely aligned ɑ-helical coil surfaces in the At EDS1- At SAG101 partner C-terminal domains that are necessary for reconstituted TNL cell death signaling. Our data suggest that TNL-triggered cell death and pathogen growth restriction are determined by distinctive features of EDS1-SAG101 and EDS1-PAD4 complexes and that these signaling machineries coevolved with other components within plant species or clades to regulate downstream pathways in TNL immunity. (© 2019 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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