| Autor: |
Matolay O; Department of Pathology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary., Beke L; Department of Pathology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary., Gyurkovics A; Department of Pathology, Jósa András Teaching County Hospital, H-4400 Nyíregyháza, Hungary., Francz M; Department of Pathology, Jósa András Teaching County Hospital, H-4400 Nyíregyháza, Hungary., Varjasi G; Hematology Unit, Jósa András Teaching County Hospital, H-4400 Nyíregyháza, Hungary., Rejtő L; Hematology Unit, Jósa András Teaching County Hospital, H-4400 Nyíregyháza, Hungary., Illés Á; Department of Hematology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary., Bedekovics J; Department of Pathology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary., Méhes G; Department of Pathology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary. gabor.mehes@med.unideb.hu. |
| Abstrakt: |
Upregulation of carbonic anhydrase IX (CAIX) was found to be associated with unfavorable prognosis and resistance to treatment in a broad spectrum of malignancies, recently also in classical Hodgkin's lymphoma (cHL). As demonstrated, variable CAIX expression in a significant number of cHL cases was associated with poor treatment response. The current study focused on the quantification CAIX immunopositivity and its relative expression compared to the total CD30+ neoplastic pool using digital image analysis. One hundred and one lymph node samples featuring cHL histology were analyzed for both CD30 and CAIX by immunohistochemistry. Whole histological slides were scanned and immunopositivity was determined as the histoscore (H-score) using the DensitoQuant software module (3DHistech Kft., Budapest, Hungary). CAIX positivity was observed in the HRS-cells of 56/101 cases (55.44%) and frequently observed in the proximity of necrotic foci. CAIX H-scores were highly variable (range: 2.16-90.36, mean 18.7 ± 18.8). Individual CAIX values were independent of the much higher CD30 values (range 3.46-151.3, mean 52.37 ± 30.74). The CAIX/CD30 index proved to be the highest in the aggressive lymphocyte-depleted (LD) subtype (CAIX/CD30: 0.876). The CAIX expression and the CAIX/CD30 relative index can be precisely determined by image analysis, and values reflect the extent of a tumor mass undergoing hypoxic-stress-related adaptation in the most aggressive forms of cHL. |
