Encapsulation of hydrophobic components in dendrimersomes and decoration of their surface with proteins and nucleic acids.

Autor:	Torre P; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058., Xiao Q; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323.; Institute of Computational Molecular Science, Temple University, Philadelphia, PA 19122., Buzzacchera I; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323.; DWI-Leibniz Institute for Interactive Materials, 52074 Aachen, Germany.; NovioSense B.V., 6534 AT Nijmegen, The Netherlands., Sherman SE; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323., Rahimi K; DWI-Leibniz Institute for Interactive Materials, 52074 Aachen, Germany.; Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany., Kostina NY; DWI-Leibniz Institute for Interactive Materials, 52074 Aachen, Germany.; Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany., Rodriguez-Emmenegger C; DWI-Leibniz Institute for Interactive Materials, 52074 Aachen, Germany.; Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany., Möller M; DWI-Leibniz Institute for Interactive Materials, 52074 Aachen, Germany.; Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, 52074 Aachen, Germany., Wilson CJ; NovioSense B.V., 6534 AT Nijmegen, The Netherlands., Klein ML; Institute of Computational Molecular Science, Temple University, Philadelphia, PA 19122; mlklein@temple.edu mattgood@pennmedicine.upenn.edu percec@sas.upenn.edu., Good MC; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058; mlklein@temple.edu mattgood@pennmedicine.upenn.edu percec@sas.upenn.edu.; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321., Percec V; Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323; mlklein@temple.edu mattgood@pennmedicine.upenn.edu percec@sas.upenn.edu.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jul 30; Vol. 116 (31), pp. 15378-15385. Date of Electronic Publication: 2019 Jul 15.
DOI:	10.1073/pnas.1904868116
Abstrakt:	Reconstructing the functions of living cells using nonnatural components is one of the great challenges of natural sciences. Compartmentalization, encapsulation, and surface decoration of globular assemblies, known as vesicles, represent key early steps in the reconstitution of synthetic cells. Here we report that vesicles self-assembled from amphiphilic Janus dendrimers, called dendrimersomes, encapsulate high concentrations of hydrophobic components and do so more efficiently than commercially available stealth liposomes assembled from phospholipid components. Multilayer onion-like dendrimersomes demonstrate a particularly high capacity for loading low-molecular weight compounds and even folded proteins. Coassembly of amphiphilic Janus dendrimers with metal-chelating ligands conjugated to amphiphilic Janus dendrimers generates dendrimersomes that selectively display folded proteins on their periphery in an oriented manner. A modular strategy for tethering nucleic acids to the surface of dendrimersomes is also demonstrated. These findings augment the functional capabilities of dendrimersomes to serve as versatile biological membrane mimics. Competing Interests: The authors declare no conflict of interest. (Copyright © 2019 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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