Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor.

Autor: Fabri-Faja N; Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, Campus UAB, 08193, Bellaterra (Barcelona), Spain., Calvo-Lozano O; Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, Campus UAB, 08193, Bellaterra (Barcelona), Spain., Dey P; Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, Campus UAB, 08193, Bellaterra (Barcelona), Spain., Terborg RA; ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain., Estevez MC; Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, Campus UAB, 08193, Bellaterra (Barcelona), Spain. Electronic address: mcarmen.estevez@icn2.cat., Belushkin A; Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Yesilköy F; Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Duempelmann L; ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain., Altug H; Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015, Switzerland., Pruneri V; ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain; ICREA-Institució Catalana de Recerca i Estudis Avançats, 08010, Barcelona, Spain., Lechuga LM; Nanobiosensors and Bioanalytical Applications Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, Campus UAB, 08193, Bellaterra (Barcelona), Spain.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2019 Oct 24; Vol. 1077, pp. 232-242. Date of Electronic Publication: 2019 May 20.
DOI: 10.1016/j.aca.2019.05.038
Abstrakt: Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical point-of-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6), and miRNAs, using miRNA-16 as an example. Limits of Detection (LoD) of 18 μg mL -1 , 88 μg mL -1 and 1 μM (6 μg mL -1 ) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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