| Autor: |
Mohammed SAE; Animal Health Research Institute, Giza 12618, Egypt., Marouf SAE; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt., Erfana AM; Animal Health Research Institute, Giza 12618, Egypt., El-Jakee JKAE; Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt., Hessain AM; Department of Health Science, College of Applied Studies and Community Service, King Saud University, P.O. Box 22459, Riyadh 11495, Saudi Arabia., Dawoud TM; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia., Kabli SA; Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia., Moussa IM; Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. |
| Abstrakt: |
Calf diarrhea is one of the major health challenges in cattle herds. The bacteriological examination of fecal samples collected from apparently healthy and diarrheic calves' revealed isolation of 26 E. coli isolates out of 56 calves with an incidence of 46.4%. Serogroups O1, O26, O44, O55, O115, O119, O125, O146, and O151 were identified from the collected fecal samples. Using PCR all isolates was positive for ompA gene species specific for E. coli . While stx 1 and eae A genes detected with incidence of 3.8 and 19.2% respectively from the isolates. The presence of stx 2 gene was negative in the fecal isolates. Among colostrum samples 4 E. coli isolates were detected and serogrouped to O26, O55 and O119. They were negative for eae A, stx 1 and stx 2 except strain number 4 (O55) was positive for stx 1. E. coli strains were sensitive to norfloxacin (80.7%) and resistant to ampicillin and cefotaxime (100% each). Based on our findings, there was no association between occurrence of E. coli and age of calf (2-14 days), while bottle feeding calf colostrum may be a source of E. coli contamination. |
