Serological evidence of Leishmania infection by employing ELISA and rapid tests in captive felids and canids in Brazil.

Autor: Tolentino N; Departamento de Medicina Veterinária, Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário, 1081, Bairro Angola, CEP 32630-000 Betim, Minas Gerais, Brazil., Pinheiro GRG; Departamento de Medicina Preventiva Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil., Ottino J; Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil., de Oliveira AR; Departamento de Clínica e Cirurgias Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil., Coelho CM; Fundação de Parques Municipais e Zoobotânica de Belo Horizonte, 31365-450 Belo Horizonte, Minas Gerais, Brazil., Tinoco HP; Fundação de Parques Municipais e Zoobotânica de Belo Horizonte, 31365-450 Belo Horizonte, Minas Gerais, Brazil., Fujiwara RT; Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil., Santos RL; Departamento de Clínica e Cirurgias Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Bairro Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil., Ribeiro VM; Departamento de Medicina Veterinária, Pontifícia Universidade Católica de Minas Gerais, Rua do Rosário, 1081, Bairro Angola, CEP 32630-000 Betim, Minas Gerais, Brazil; Santo Agostinho Hospital Veterinário, Avenida Amazonas, 2218, Santo Agostinho, Belo Horizonte, Minas Gerais 30180-003, Brazil. Electronic address: ribeirovm@yahoo.com.br.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2019 Aug; Vol. 17, pp. 100308. Date of Electronic Publication: 2019 May 29.
DOI: 10.1016/j.vprsr.2019.100308
Abstrakt: Visceral leishmaniasis (VL) is a zoonosis and the dog is considered the most important urban reservoir. Cases in cats have been reported, but little is known about Leishmania infection and disease in wild felids and canids kept in captivity in endemic areas. Thus, the serological pattern of wild felids and canids kept in captivity at the Belo Horizonte Zoological Garden was investigated using two primary antigens for conventional ELISA: k39 and rKDDR, as well as three serological rapid kits: Dual Path Platform (DPP®) immunochromatographic test, rKDDR Immunochromatographic assay and ELISA SNAP Leishmania IDEXX®. A total of 21 serum samples, 13 of wild felids and 8 wild canids of varying age and sex were evaluated. The results obtained in the tests were analyzed by agreement using Kappa coefficient, and between ELISA antigens all the analysis performed had showed significant agreement among both of them, as well between the three immunochromatographic tests. The results demonstrated that there is serological evidence of wild animals seropositive for Leishmania antibodies at the Belo Horizonte Zoological Garden, and that all the antigens and rapid tests used can be employed in serological screening for VL in wild felids and canids.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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