Balanced synaptic currents underlie low-frequency oscillations in the subiculum.

Autor: Royzen F; Interdepartmental Program in Neuroscience, University of Utah, Salt Lake City, Utah.; Department of Biomedical Engineering, Boston University, Boston, Massachusetts., Williams S; Department of Psychiatry, McGill University, Montreal, Quebec, Canada., Fernandez FR; Department of Biomedical Engineering, Center for Systems Neuroscience, Boston University, Boston, Massachusetts., White JA; Department of Biomedical Engineering, Center for Systems Neuroscience, Boston University, Boston, Massachusetts.
Jazyk: angličtina
Zdroj: Hippocampus [Hippocampus] 2019 Dec; Vol. 29 (12), pp. 1178-1189. Date of Electronic Publication: 2019 Jul 13.
DOI: 10.1002/hipo.23131
Abstrakt: Numerous synaptic and intrinsic membrane mechanisms have been proposed for generating oscillatory activity in the hippocampus. Few studies, however, have directly measured synaptic conductances and membrane properties during oscillations. The time course and relative contribution of excitatory and inhibitory synaptic conductances, as well as the role of intrinsic membrane properties in amplifying synaptic inputs, remains unclear. To address this issue, we used an isolated whole hippocampal preparation that generates autonomous low-frequency oscillations near the theta range. Using 2-photon microscopy and expression of genetically encoded fluorophores, we obtained on-cell and whole-cell patch recordings of pyramidal cells and fast-firing interneurons in the distal subiculum. Pyramidal cell and interneuron spiking shared similar phase-locking to local field potential oscillations. In pyramidal cells, spiking resulted from a concomitant and balanced increase in excitatory and inhibitory synaptic currents. In contrast, interneuron spiking was driven almost exclusively by excitatory synaptic current. Thus, similar to tightly balanced networks underlying hippocampal gamma oscillations and ripples, balanced synaptic inputs in the whole hippocampal preparation drive highly phase-locked spiking at the peak of slower network oscillations.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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