Control of bacterial and fungal biofilms by natural products of Ziziphus joazeiro Mart. (Rhamnaceae).
Autor: | Cosmo Andrade J; Laboratory of Microbiology and Molecular Biology - LMBM, University Regional of Cariri - URCA, Crato, CE Brazil; Laboratory Bioassay - LABIO, University Federal of Cariri - UFCA, Institute of Educators Training, Campus Brejo Santo, CE, Brazil., da Silva ARP; Laboratory of Microbiology and Molecular Biology - LMBM, University Regional of Cariri - URCA, Crato, CE Brazil., Audilene Freitas M; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., de Azevedo Ramos B; Center of Biological Sciences and Department of Biochemistry, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Sampaio Freitas T; Laboratory of Microbiology and Molecular Biology - LMBM, University Regional of Cariri - URCA, Crato, CE Brazil., de Assis G Dos Santos F; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Leite-Andrade MC; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Nunes M; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Relison Tintino S; Laboratory of Microbiology and Molecular Biology - LMBM, University Regional of Cariri - URCA, Crato, CE Brazil., da Silva MV; Center of Biological Sciences and Department of Biochemistry, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Dos Santos Correia MT; Center of Biological Sciences and Department of Biochemistry, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., de Lima-Neto RG; Department of Tropical Medicine, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Neves RP; Laboratory of Medical Mycology Sylvio Campos, University Federal of Pernambuco - UFPE, Recife, PE, Brazil., Melo Coutinho HD; Laboratory of Microbiology and Molecular Biology - LMBM, University Regional of Cariri - URCA, Crato, CE Brazil. Electronic address: hdmcoutinho@gmail.com. |
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Jazyk: | angličtina |
Zdroj: | Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2019 Aug; Vol. 65, pp. 226-233. Date of Electronic Publication: 2019 Jun 17. |
DOI: | 10.1016/j.cimid.2019.06.006 |
Abstrakt: | The aim of this study is to verify the action of the aqueous leaf extract Ziziphus joazeiro in the eradication of bacterial and fungal biofilms, and to compare these effects with the stem bark extracts, as well as with conventional standard drugs. The presence of secondary metabolites was observed through phytochemical prospection assays. The effect of the aqueous extract on microbial biofilm formation was observed by OD600 nm absorbance and the crystal violet assay. For bacterial and fungal biofilms, chlorhexidine gluconate and fluconazole, respectively, were used as positive controls. Phytochemical characterization showed the presence of secondary metabolite classes common to both extracts such as flavonoids, steroids and saponins. In particular, in the aqueous leaf extract phenols, condensed tannins and alkaloids were observed. Eradication results using the aqueous leaf extract showed an inhibition of the microbial biofilm mass, moreover the biofilms were more sensitive to the bark extract, which presented a greater inhibition number and an action similar to standard drugs. It is important to highlight the leaf extract showed significant eradication at the lowest concentrations for mature yeast biofilms, thus demonstrating its potential to modify microbial resistance susceptibility. Bacterial and fungal biofilm eradication results using the Ziziphus joazeiro aqueous extracts presented a biofilm inhibition effect for both, moreover the results support the ethnopharmacological knowledge surrounding the use of Ziziphus joazeiro stems in the community. In comparison, the bark extract presented a more effective treatment than the leaf extract against biofilms, presenting inhibition levels similar to the used standard drugs. (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
Databáze: | MEDLINE |
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