Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization.

Autor: Kolomytseva MP; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia. Electronic address: mkolomytseva@rambler.ru., Myasoedova NM; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Chernykh AM; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Gaidina AS; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Shebanova AD; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Baskunov BP; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Aschenbrenner J; Institute of Bio- and Geosciences IBG-1: Bioorganic Chemistry, Forschungszentrum Jülich GmbH, Germany., Rosengarten JF; Institute of Bio- and Geosciences IBG-1: Bioorganic Chemistry, Forschungszentrum Jülich GmbH, Germany., Renfeld ZV; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Gasanov NB; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Pinchuk IP; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia., Classen T; Institute of Bio- and Geosciences IBG-1: Bioorganic Chemistry, Forschungszentrum Jülich GmbH, Germany., Pietruszka J; Institute of Bio- and Geosciences IBG-1: Bioorganic Chemistry, Forschungszentrum Jülich GmbH, Germany; Institute for Bioorganic Chemistry, Heinrich Heine University Düsseldorf, Germany., Golovleva LA; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Sep 15; Vol. 137, pp. 1199-1210. Date of Electronic Publication: 2019 Jul 08.
DOI: 10.1016/j.ijbiomac.2019.07.056
Abstrakt: Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59, 65, and 61 kDa respectively) were more active in acidic conditions at around 70 °C. However, in contrast to laccases I and II, laccase III retained its activity (8-30%) and stability during at least one week of incubation at neutral conditions that allows its biotechnological application carried out at neutral environment. The activation phenomena for some of the purified laccases from L. strigosus 1566 during incubation at high temperature, different pH, and sulfates is shown and discussed. According to MALDI-TOF analysis, laccases I and II are most closely related to the laccase of Panus rudis (AAR13230). Transformation of phenylpropanoids by the predominant laccases of L. strigosus 1566 to different polymers was demonstrated, indicating a great potential for producing novel pharmaceutical valuable analogues of lignans, stilbenes, flavonoids, and etc.. The studied laccases, which are products of the same strain, can become a convenient model for further studies of the structural mechanisms of the shift of T-/pH-optima, activation, and T-/pH-stability.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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