Living specimens under field immobilization and smartphone microscopic observation.

Autor: Ahmad Zahidi AA; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, Australia., Chung DCK; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, Australia., Katariya M; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, Australia., Liew OW; Centre for Translational Medicine, Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore., Ng TW; Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria, Australia.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2019 Oct; Vol. 82 (10), pp. 1741-1747. Date of Electronic Publication: 2019 Jul 11.
DOI: 10.1002/jemt.23340
Abstrakt: In this work a single glycerol-water mixture, determined to be most apt at 30% (wt/vol), was used to immobilize Drosophila samples as well as to create a liquid lens for smartphone magnification viewing. This provides the advantage of being able to observe immobilized insects directly in the field rather than in the laboratory. In order to avoid having bubbles in the liquid lens and immobilizing medium that hinder visibility, an approach was developed where a stable pendant drop is moved in tandem with the dispensing tip by gravity and stopped abruptly so that sufficient momentum is transferred to the drop for its dislodgement. With 30% glycerol-water (wt/vol) mixtures, applying a minimal stroke of 10 mm with longitudinal impact delivered a momentum of 0.1464 N/s that allowed transfer of a preselected liquid volume for the processes.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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